Metabolomic profiling in ankylosing spondylitis using time-of-flight mass spectrometry

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dc.authoridDOGAN, Halef Okan/0000-0001-8738-0760
dc.contributor.authorDogan, Halef Okan
dc.contributor.authorSenol, Onur
dc.contributor.authorKaradag, Ahmet
dc.contributor.authorYildiz, Seyma Nur
dc.date.accessioned2024-10-26T18:09:25Z
dc.date.available2024-10-26T18:09:25Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground & aims: Ankylosing spondylitis (AS) is an inflammatory disease associated with destructive changes in the skeleton and joints. The exact molecular mechanism of the disease has not been fully elucidated. This study aimed to determine metabolic differences between active AS patients and healthy controls to understand the molecular mechanism of AS. Patients and methods: The study included 38 subjects, comprising 18 patients with active AS and 20 healthy controls. Metabolic profiling of the plasma was performed using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS). Data acquisition, classification, and identification were achieved with the METLIN (https://metlin.scripps.edu/) database and XCMS (https://xcmsonline.scripps.edu). Results: Significant alterations were identified in the unsaturated fatty acids (FA), linoleic acid, alphalinolenic acid, FA degradation, and FA biosynthesis pathways. Down -regulations were observed in phosphatidylcholine (PC) (16:0/0:0), beta-D-Fructose, stearic acid, trimipramine N-Oxide and muconic acid, and up-regulation were detected in PC (18:2/0:0), 3-Methylindole, palmitic acid (PA), alphaTocotrienol, and beta-D-glucopyranoside in active AS patients compared to the healthy control subjects. Conclusion: Pathway analysis revealed that dysregulation in FA metabolism is associated with AS, and therefore, modulation of diet according to PA and PC may be potential therapeutic targets. (C) 2022 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved.
dc.description.sponsorshipSivas Cumhuriyet University, Turkey [T-883, RGD-036]
dc.description.sponsorshipThe study was supported by Sivas Cumhuriyet University, Turkey with the grant numbers of T-883 and RGD-036.
dc.identifier.doi10.1016/j.clnesp.2022.06.011
dc.identifier.endpage132
dc.identifier.issn2405-4577
dc.identifier.pmid35871913
dc.identifier.startpage124
dc.identifier.urihttps://doi.org/10.1016/j.clnesp.2022.06.011
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30091
dc.identifier.volume50
dc.identifier.wosWOS:000852860200016
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofClinical Nutrition Espen
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnkylosing spondylitis
dc.subjectFatty acid
dc.subjectMetabolomics
dc.subjectPhosphatidylcholine
dc.subjectUPLC Q-TOF/MS
dc.subjectPlasma
dc.titleMetabolomic profiling in ankylosing spondylitis using time-of-flight mass spectrometry
dc.typeArticle

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