An Isolate of Bacillus clausii Appears to Possess Four Distinct Pectinolytic Activities

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dc.contributor.authorBerber, Sevinç
dc.contributor.authorÇetinkaya, Serap
dc.date.accessioned2024-10-26T17:38:03Z
dc.date.available2024-10-26T17:38:03Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractFor the bacterial source, Koyulhisar (Sivas, Turkey) apple orchard soil samples were used. Obtained isolates were identified at the species level by partial protein- and 16S rRNA gene sequence homology All of them were determined to be Bacillus clausii. Cultures were prepared with these isolates and after removing the cells, the proteins in the upper fluid were precipitated with the help of an organic solvent. While performing pectinase screening, it was noticed that an existing protease digested other proteins. This proteolytic activity was eliminated by increasing the incubation temperature to 55oC. Four different pectinase activities, polygalacturonase, pectin lyase, pectate lyase, and pectin methylesterase, were then demonstrated. Four protein bands corresponding to these activities visualised by SDS-PAGE were 80 kDa, 58 kDa, 46 kDa and ~ 28 kDa, respectively. Spectroscopic methods were employed for the identification of the enzymes. The smallest protein band (28 kDa) was found to be pectin methylesterase. Km and Vmax values of this enzyme were 0.4 and 217U, respectively.
dc.identifier.doi10.21597/jist.768556
dc.identifier.endpage644
dc.identifier.issn2146-0574
dc.identifier.issn2536-4618
dc.identifier.issue1
dc.identifier.startpage635
dc.identifier.trdizinid1143979
dc.identifier.urihttps://doi.org/10.21597/jist.768556
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1143979
dc.identifier.urihttps://hdl.handle.net/20.500.12418/23810
dc.identifier.volume11
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofIğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacillus clausii
dc.subjectpectin lyase
dc.subjectpolygalacturonase
dc.subjectpectate lyase
dc.subjectpectin methylesterase
dc.titleAn Isolate of Bacillus clausii Appears to Possess Four Distinct Pectinolytic Activities
dc.typeArticle

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