Investigation of RN7SL1 Expression in Human Umbilical Vein Endothelial Cells After Oxidized Low-Density Lipoprotein Stimulation: Experimental Study

Objective: Oxidized low-density lipoprotein (ox-LDL) dam-ages endothelial cells (ECs) and induces pathogenic processes related to atherosclerosis (AS). Long non-coding RNAs (lncRNAs) have been accepted to be transcripts code no proteins and have important roles in gene expression regulation. LncRNAs have been thought important targets in the fight against cardiovascular diseases (CVDs). RNA component of signal recognition particle (SRP) 7SL1 (RN7SL1) is a lncRNA. RN7SL1 was associated with inhibition of cell cycle arrest. Since RN7SL1 is associated with cell cycle arrest and ox-LDL affects EC cell apoptosis, hypothesis of our study is that ox-LDL may alter the expression of this lncRNA in ECs. There was no study yet on RN7SL1 and CVDs including AS. We aimed to compare RN7SL1 gene expression between ECs treated with ox-LDL and normal ECs. Material and Methods: We investigated expression level of lncRNA RN7SL1 in human umbilical vein endothelial cells (HUVECs) induced with ox-LDL in this study. We performed measurement with SYBR green dye using quantitative PCR (real-time PCR, qPCR) method. Results: We found that expression of RN7SL1 was up-regulated 6.06 fold statistic significantly in HUVECs after ox-LDL treatment (P<0.001**). RN7SL1 up-regulation may modulate EC cycle and apoptosis in AS, since ox-LDL stimulation increased expression of RN7SL1 gene in HUVECs. Conclusion: According to findings of the current study, our interest focused on that RN7SL1 may associated with vascular damage of the ECs at initation of the AS. Our results further will guide future studies related to the RN7SL1 and CVDs including AS. © 2025 by Türkiye Klinikleri.