Assessment of Mineral Content Variations for Biofortification of the Bean Seed

Germplasm collections are very important for breeder to develop new cultivars with highmineral nutrients and yield. Eighty-three Phaseolus landraces were collected from different provincesof Western Anatolia Region of Turkey in 2015-2016. Twenty common bean lines were selectedaccording to morphological characterization results and weighted scaling method in 2016. Phosphorus(P), potassium (K), copper (Cu), zinc (Zn), manganese (Mn), iron (Fe), calcium (Ca), and magnesium (Mg)contents of these twenty common bean lines and two commercial cultivars were tested under fieldconditions. Randomized block design with three replicates was used for analysis in 2017 growingseason on the experimental farm of Bolu Abant Izzet Baysal University. The results showed high levelof variation among lines and cultivars in terms of P (0.94-1.30%), K (2.38-3.59%), Cu (7.80-14.80 mgkg-1), Zn (19.74-66.68 mg kg-1), Mn (7.46-27.25 mg kg-1), Fe (48.98-182.45 mg kg-1), Ca (0.18-0.48 mgkg-1) and Mg (0.56-0.71 mg kg-1) contents. Positive correlations were found between K and Zn (r=0.447;P<0.05), P and Fe (r=0.485; P<0.05), Ca and Mg (r=0.693; P<0.01). In principal component analysis(PCA), the first 4 principal components accounted for approximately 73% of the total variability. Thelines, Ylv-14, Ylv-32, Blck-7, Blksr-3 and Brs-22 had superior mineral contents for Fe and P, Cu and Mn,Ca and Mg, Zn, and K, respectively. Therefore, these lines represent promising candidates forbiofortifying the bean seed and can be registered as cultivars in Turkey. Moreover, these lines will beused further for identifying the QTL regions by developing biparental mapping populations for aneffective breeding program in Turkey in near future.