Effects of metformin and ganirelix on subcutaneous endometriosis in a mouse model of autophagy-related cell death

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dc.contributor.authorÜnal, Gamze Sönmez
dc.contributor.authorAlbak, Yasemin
dc.contributor.authorYurtcu, Nazan
dc.contributor.authorYıldız, Çağlar
dc.contributor.authorÇetin, Meral
dc.contributor.authorDaştan, Sevgi Durna
dc.contributor.authorGümüş, Erkan
dc.date.accessioned2024-10-26T17:42:40Z
dc.date.available2024-10-26T17:42:40Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjective: This study aimed to investigate the efficacy of metformin and ganirelix on subcutaneous endometriotic tissues created in an experimental mouse model. Materials and Methods: Five groups were formed with eight animals in each group. One of the groups was set as the control group. Endometriotic lesions were created by transplanting 40 mouse autologous endomyometrial tissues into the mouse subcutaneous tissue to a highly vascular surface. Gene expression analyzes of tissues were performed as HIF-1a, ATG5, ATG12, Beclin2, Beclin1, LC3BII, CateninB, GSK3b, TCF, WNT2, WNT7a, and WNT10a gene analyzes. Drug effects were examined by histological examination. HIF1a and WNT2 protein expressions were examined immunohistochemically. Gene expression coefficients of control, metformin day 1 (Met1g), metformin day 7 (Met7g), ganirelix day 1 (Gnx1g), and ganirelix day 7 (Gnx7g) groups are shown in tables. Data are presented as mean and standard error. Results: Beclin2 gene expression coefficients of metformin 1st day, metformin 7th day, ganirelix 1st day, and general 7th day groups were found to have significantly decreased compared with the control group coefficient. Beclin1 gene expression coefficients of metformin 1st day, metformin 7th day, ganirelix 1st day, and genirelix 7th day groups were found to have significantly decreased compared with the control group coefficient. LC3BII gene expression coefficients of metformin 1st day and metformin 7th day groups were found to have significantly decreased compared withLC3BII gene expression coefficients of control, genirelix 1st day, and genirelix 7th day groups. These findings were supported by histological and immunohistochemical staining. Conclusion: These genes are actively involved in the autophagy pathway, and we think that the use of metformin in endometriosis might create an autophagy-based suppression mechanism.
dc.identifier.doi10.4274/tjod.galenos.2023.85616
dc.identifier.endpage226
dc.identifier.issn2149-9322
dc.identifier.issn2149-9330
dc.identifier.issue3
dc.identifier.startpage219
dc.identifier.trdizinid1242410
dc.identifier.urihttps://doi.org/10.4274/tjod.galenos.2023.85616
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1242410
dc.identifier.urihttps://hdl.handle.net/20.500.12418/24755
dc.identifier.volume20
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTurkish Journal of Obstetrics and Gynecology
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMetformin
dc.subjectEndometriosis
dc.subjectganirelix
dc.subjectsubcutaneous endometriosis
dc.subjectmouse model
dc.titleEffects of metformin and ganirelix on subcutaneous endometriosis in a mouse model of autophagy-related cell death
dc.typeArticle

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