Investigation of yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss Walbaum 1792) farms by polymerase chain reaction (PCR) and bacteriological culture

Basit Öğe Kaydı
dc.contributor.authorŞeker, Engin
dc.contributor.authorKarahan, Murat
dc.contributor.authorIspir, Ünal
dc.contributor.authorÇetinkaya, Burhan
dc.contributor.authorSa?lam, Naim
dc.contributor.authorSarieyyüpo?lu, Mustafa
dc.date.accessioned2024-10-26T17:54:10Z
dc.date.available2024-10-26T17:54:10Z
dc.date.issued2012
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study has been carried out to investigate the presence of Yersinia ruckeri, the causative agent of Enteric Red Mouth (ERM) disease, in rainbow trout fish breeding farms located in Elazig and Malatya provinces in Eastern Turkey. For this purpose, bacteriological culture and a specific polymerase chain reaction (PCR) have been compare with examine blood and intestine samples collected from adult and young rainbow trout fish as well as water samples collected from all the farms. Y. ruckeri was isolated and identified from eight of the 17 fish farms giving a proportion of 52.9% at fish farm. The disease was noted in five of the rainbow trout farms located in Elazig and three of the 11 rainbow trout farms located in Malatya. The agent was detected in five of the six young fish breeding farms. The isolation percentages of the causative agent at fish level were calculated as 25.7% (131/510) in adult and 31.7% (19/60) in young fish, giving an overall proportion of 26.3% (150/570). All the isolates have been successfully identified as Y. ruckeri by species-specific PCR. No isolation could be made from water samples. It is therefore concluded that Y. ruckeri infection poses a significant threat to the fish farms in the study area and necessary precautions should urgently be taken in order to minimize economical losses due to ERM disease. A specific PCR can be utilized successfully in aquaculture for rapid identification of bacterial agents which will help to prevent spread of infectious diseases and will therefore contribute to the productivity of fishery sector.
dc.identifier.endpage916
dc.identifier.issn1300-6045
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84866764971
dc.identifier.scopusqualityQ3
dc.identifier.startpage913
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27022
dc.identifier.volume18
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherVeteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCulture; PCR; Rainbow trout; Yersinia ruckeri
dc.titleInvestigation of yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss Walbaum 1792) farms by polymerase chain reaction (PCR) and bacteriological culture
dc.title.alternativeGökkuşa?ı alabalı?ı (Oncorhynchus mykiss Walbaum 1792) çiftliklerinde yersinia ruckeri i?nfeksiyonunun bakteriyolojik kültür ve polimeraz zincir reaksiyonu (PCR) ile i?ncelenmesi
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu