Effect of the wrapping of experimentally induced ischemic colonic anastomoses with the amniotic membrane on anastomosis safety in rats.

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dc.contributor.authorSoylu, Sinan
dc.contributor.authorİnan, Zeynep Deniz Şahin
dc.date.accessioned2024-10-26T17:44:19Z
dc.date.available2024-10-26T17:44:19Z
dc.date.issued2018
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjective: Colonic anastomotic leakage represents a complication with high mortality and morbidity. In the presentstudy, it was aimed to examine the effect of the amniotic membrane on the healing of ischemic colonic anastomosis.Method: 25 pregnant Wistar albino rats aged 19-21 days were randomly divided into 5 groups. With midlinelaparotomy, pregnancies were terminated, and fresh amniotic membranes were obtained. In Group 1 Sham (S), noprocedure except for laparotomy was performed. In Group 2 of colonic anastomosis (A), the descending colon wascompletely transected and anastomosed. In Group 3 of amniotic anastomosis (AA), the anastomosis line was fixed bybeing wrapped with the fresh amniotic membrane. In Group 4 of colonic ischemic anastomosis (IA), the marginalarteries were ligated after descending colon transection. Ischemia was induced, and anastomosis was performed. InGroup 5 of colonic ischemic anastomosis wrapped with the amniotic membrane (AIA), after ischemic colonicanastomosis was performed, the anastomosis line was fixed by being wrapped with the amniotic membrane. On thepostoperative 7th day, rats were sacrificed, and their anastomotic bursting pressures were measured. For histologicalexamination, the colon segment containing the anastomosis line was removed. Hematoxylin-eosin and Van Giesontrichrome stained specimens were histologically evaluated and scored according to the Ehrlich-Hunt model.Results: Anastomotic bursting pressures were measured in group S as the highest, in AA, AIA, A in a decreasing orderand in group IA as the lowest. The difference between groups S and AA was statistically insignificant in the pairedcomparison of groups according to their bursting pressure values (p=0.222). When the other pairs were compared, thedifference between the groups was calculated as significant (p< 0.05). According to the Ehrlich-Hunt model, the totalscore was the highest in AIA group, decreased in AA, IA, A groups in a decreasing order, and was the lowest in groupS. A paired comparison for total scores between the groups was statistically insignificant between groups S and A(p=0.222). Other paired comparisons were statistically significant (p< 0.05).Conclusions: Wrapping the colon anastomosis line with the amniotic membrane gave positive results both in theischemic colon as well as in the non-ischemic colon in terms of bursting pressure and histological anastomosis safety.
dc.identifier.doi10.7197/223.vi.460562
dc.identifier.endpage298
dc.identifier.issn1305-0028
dc.identifier.issue3
dc.identifier.startpage291
dc.identifier.trdizinid326294
dc.identifier.urihttps://doi.org/10.7197/223.vi.460562
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/326294
dc.identifier.urihttps://hdl.handle.net/20.500.12418/25201
dc.identifier.volume40
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCumhuriyet Tıp Dergisi (ELEKTRONİK)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCerrahi
dc.titleEffect of the wrapping of experimentally induced ischemic colonic anastomoses with the amniotic membrane on anastomosis safety in rats.
dc.typeArticle

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