Protective Effects of Lactobacillus plantarum Exopolysaccharides from Home Made Dairy Products on Obesity

As it is known, obesity is one of the most important health problems of developed and developing countries today. Obesity; endocrine, cardiovascular, respiratory, gastrointestinal (GI), genitourinary, musculoskeletal system such as body systems and psychosocial condition causes many health problems due to the negative effects. The ability of probiotics to support GI health has been recognized for years. Since alternative approaches against obesity can be developed by intervention in the GI system, this study aimed to evaluate the effects of exopolysaccharides (EPS) as an anti-obesity biomaterial isolated from natural products with probiotic characteristics. First, the one with the highest EPS amount among the 10 microorganisms with known probiotic properties was identified by MALDI-TOF and 16s rRNA sequence analysis and named as Lactobacillus plantrum BHC007. Then, the monosaccharide composition of the extracted EPS was examined, and its average molecular weight was determined as 221.860±0.006. The effect of EPS on the adipogenesis process was examined qualitatively with Oil Red O dye on the 3T3 cell line and cellular triacylglycerol (TAG) levels. It was concluded that the highest EPS concentration (2.25 mM) inhibited adipogenesis by 75%. Also, 17% inhibition of adipogenesis was achieved at the lowest EPS concentration (0.45 mM). In conclusion, TAG analysis revealed that EPS was suppressed depending on the lipid droplet formation concentration.

[No abstract available]