Effects of experimental wheat and corn gluten on liver tissue in rats: biochemical, histopathological, immunohistochemical and immunofluorescence methods

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dc.contributor.authorKapakin, Kübra Asena Terim
dc.contributor.authorGümüş, Recep
dc.contributor.authorBolat, İsmail
dc.contributor.authorKirman, Esra Manavoğlu
dc.contributor.authorİmik, Aybüke
dc.date.accessioned2025-05-04T16:41:53Z
dc.date.available2025-05-04T16:41:53Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis study was aimed at determining the effects of wheat gluten, corn gluten and soybean meal, incorporated into feed as protein sources, on the hepatic tissue of rats, based on the investigation of histopathological parameters (degeneration, inflammation, biliary hyperplasia and fat droplets), immunohistochemical parameters (transglutaminase, gliadin, IgA, IgG, CD4 and CD8), and the serum levels of hepatic enzymes [aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH)]. Three groups, referred to as Wheat Group, Group Corn and Group Soybean, were established, and were given high levels of wheat gluten, corn gluten and soybean meal as protein sources in the feed, respectively. The study material comprised forty–eight 20–day–old female Sprague Dawley rats. In the group, which received dietary wheat gluten, the hepatic tissue presented with numerically higher histopathological and immunohistochemical parameters on day 45 of the study, and numerically higher histopathological parameters on day 165 of the study (P>0.05). On day 165 of the study, when compared to Groups Corn and Soybean, Group Wheat displayed a significantly different level of sensitivity to immunohistochemical parameters (transglutaminase, gliadin, IgA, IgG, CD4 and CD8). It was determined that gluten significantly reduced the activity of the liver enzymes LDH and AST. In result, it was ascertained that rats, not carrying the genes HLA–DQ2 and DQ8, when fed on a ration incorporated with a high level of wheat gluten, displayed slightly affected hepatic tissue histopathological parameters and significantly increased immunohistochemical parameters (transglutaminase, gliadin, IgA, IgG, CD4 and CD8). © (2024), (Universidad del Zulia). All Rights Reserved.
dc.identifier.doi10.52973/RCFCV-E34500
dc.identifier.issn0798-2259
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85217453889
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.52973/RCFCV-E34500
dc.identifier.urihttps://hdl.handle.net/20.500.12418/34949
dc.identifier.volume34
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUniversidad del Zulia, Facultad de Ciencias Veterinarias
dc.relation.ispartofRevista Cientifica de la Facultad de Veterinaria
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250504
dc.subjectcorn
dc.subjectgluten
dc.subjectimmunohistochemical
dc.subjectRat
dc.subjectwheat
dc.titleEffects of experimental wheat and corn gluten on liver tissue in rats: biochemical, histopathological, immunohistochemical and immunofluorescence methods
dc.title.alternativeEfectos del gluten de trigo y maíz experimental sobre el tejido hepático de ratas: métodos bioquímicos, histopatológicos, inmunohistoquímicos y de inmunofluorescencia
dc.typeArticle

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