Tarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats

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dc.contributor.authorAkcakavak, Gokhan
dc.contributor.authorCelik, Zeynep
dc.contributor.authorKaratas, Ozhan
dc.contributor.authorDogan, Osman
dc.contributor.authorOzdemir, Ozgur
dc.contributor.authorTuzcu, Mehmet
dc.date.accessioned2024-10-26T17:53:04Z
dc.date.available2024-10-26T17:53:04Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractPurpose: To evaluate the effect of a combination of Tarantula cubensis alcohol extract (TCAE) and capecitabine (CAP) in the treatment of azoxymethane (AOM)-induced colorectal cancer (CRC). Methods: Forty-two Wistar albino rats were divided into 7 groups with 6 rats in each group. The groups consisted of Control (C), Control+TCAE (C-TCAE), Control+CAP (C-CAP), Cancer control (CC), Cancer+TCAE (CC-TCAE), Cancer+CAP (CC-CAP) and Cancer+CAP+TCAE (CC-CAP+TCAE). To induce CRC, AOM (15 mg/kg) was administered to rats subcutaneously (sc) twice at a one-week interval to all the groups except control. From the 15th week, TCAE (0.2 mL/rat sc) was administered to CC-TCAE group every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered by gavage to CC-CAP group for 4 weeks. In CC-CAP+TCAE group, TCAE (0.2 mL/rat sc) was administered every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered gavage for 4 weeks. Animals were treated for 18 weeks. Aberrant crypt foci (ACF) were evaluated histopathologically among CC, CC-TCAE, CC-CAP, and CC-CAP+TCAE groups. ?-catenin, CD15, Proliferating Cell Nuclear Antigen (PCNA), and Nuclear Factor kappa B (NF-?B) expression levels were immunohistochemically compared among all groups. Results: Histopathologically, ACF scores were significantly increased in CC group, while a significant decrease in the relevant scores (p < 0.001) was observed in CC-CAP and CC-CAP+TCAE treatment groups, and the lowest scores were in CC-CAP+TCAE group. Immunohistochemically, in CC group, ?-catenin, Nuclear Factor kappa B (NF-?B), Proliferating Cell Nuclear Antigen (PCNA) and CD15 expressions were highly irregular. CC-CAP and CC-CAP+TCAE groups had significantly reduced expressions (p < 0.001), and the lowest expressions were in CC-CAP+TCAE group. Conclusion: The combined use of TCAE and CAP in treatment of CRC has a synergistic effect and increases the anticancer efficacy of TCAE, and CAP. More studies at the molecular level are needed in the future to demonstrate the clinical benefit of TCAE supplementation during the treatment of CRC with CAP. © 2024 The authors. This work is licensed under the Creative Commons Attribution 4.0 International License.
dc.identifier.doi10.4314/tjpr.v23i2.8
dc.identifier.endpage297
dc.identifier.issn1596-5996
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85188745206
dc.identifier.scopusqualityQ3
dc.identifier.startpage291
dc.identifier.urihttps://doi.org/10.4314/tjpr.v23i2.8
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26675
dc.identifier.volume23
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUniversity of Benin
dc.relation.ispartofTropical Journal of Pharmaceutical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAzoxymethane; CD15; Colorectal cancer; NF-?B; PCNA; ?-catenin
dc.titleTarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
dc.typeArticle

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