New Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme

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dc.contributor.authorAkkaya, Birnur
dc.contributor.authorAkkaya, Recep
dc.date.accessioned2024-10-26T17:34:06Z
dc.date.available2024-10-26T17:34:06Z
dc.date.issued2018
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIn this study, we investigated adsorption behaviour of lysozyme on poly(hydroxyethylmethacrylateexpanded perlite) [P(Hema-Ep)]. For this purpose, we synthesized P(Hema-Ep) composite by bulk polymerization and it was characterized by SEM, FTIR and swelling ratio. The fresh chicken egg white lysozyme was used as a model protein, had an isoelectric point (pI) 10.7 and molecular weight (MW) 14 kDa. Adsorption capacity was found 51 mg g-1 at room temperature and in 0.1 M pH=8.0 phosphate buffer. Experiments on desorption and reusability were also performed. It appears that P(Hema-Ep) composites can be used ten times without lost of efficiency. Lysozyme purification, without causing any denaturation, can be performed from chicken egg white by using this new synthesized composite in a single step.
dc.identifier.doi10.17776/csj.423415
dc.identifier.endpage702
dc.identifier.issn2587-2680
dc.identifier.issn2587-246X
dc.identifier.issue3
dc.identifier.startpage694
dc.identifier.trdizinid302526
dc.identifier.urihttps://doi.org/10.17776/csj.423415
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/302526
dc.identifier.urihttps://hdl.handle.net/20.500.12418/23516
dc.identifier.volume39
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCumhuriyet Science Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiyoloji
dc.titleNew Composite, Poly(Hydroxyethylmethacrylate-Expanded Perlite), for Single step Separation of Egg White Lysozyme
dc.typeArticle

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