Comparison of tuberculin skin test, ifn-? assay, real time pcr and lateral flow rapid test in diagnosis of field outbreaks of bovine tuberculosis

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dc.contributor.authorKaya, Abdullah
dc.contributor.authorİçen, Hasan
dc.contributor.authorTuzcu, Nevin
dc.contributor.authorŞimşek, Aynur
dc.contributor.authorYeşilmen, Simten
dc.contributor.authorKoçhan, Akın
dc.contributor.authorYildirim, İbrahim
dc.date.accessioned2024-10-26T17:53:14Z
dc.date.available2024-10-26T17:53:14Z
dc.date.issued2015
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-?) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-? assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-? assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-? assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey. © 2015 Veteriner Fakultesi Dergisi. All rights reserved.
dc.identifier.doi10.9775/kvfd.2015.13293
dc.identifier.endpage743
dc.identifier.issn1300-6045
dc.identifier.issue5
dc.identifier.scopus2-s2.0-84934759998
dc.identifier.scopusqualityQ3
dc.identifier.startpage739
dc.identifier.urihttps://doi.org/10.9775/kvfd.2015.13293
dc.identifier.urihttps://hdl.handle.net/20.500.12418/26776
dc.identifier.volume21
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherVeteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIFN-? assay; Real time PCR; TB lateral flow rapid test; Tuberculin skin test; Tuberculosis
dc.titleComparison of tuberculin skin test, ifn-? assay, real time pcr and lateral flow rapid test in diagnosis of field outbreaks of bovine tuberculosis
dc.title.alternativeSahada Görülen Sığır Tüberkülozunun Tanısında Kullanılan Tuberculin Skin Test, IFN-?  ssay, Gerçek Zamanlı PCR ve Lateral Flow Rapid Testlerinin Karşılaştırılması
dc.typeArticle

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