Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1

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dc.contributor.authorErol, Ufuk
dc.contributor.authorSahin, Omer Faruk
dc.contributor.authorUrhan, Osman Furkan
dc.contributor.authorAtas, Ahmet Duran
dc.contributor.authorAltay, Kursat
dc.date.accessioned2024-10-26T18:05:16Z
dc.date.available2024-10-26T18:05:16Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAnaplasma phagocytophilum is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Turkiye were researched for A.phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum-like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Turkiye and around the world. A.phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.
dc.description.sponsorshipScientific Research Project Fund of Sivas Cumhuriyet University [V-2022-127]
dc.description.sponsorshipThis work is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number V-2022-127.
dc.identifier.doi10.1016/j.cimid.2024.102154
dc.identifier.issn0147-9571
dc.identifier.issn1878-1667
dc.identifier.pmid38442543
dc.identifier.urihttps://doi.org/10.1016/j.cimid.2024.102154
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28899
dc.identifier.volume107
dc.identifier.wosWOS:001203731400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofComparative Immunology Microbiology and Infectious Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectA. phagocytophilum-like 1
dc.subjectTurkiye
dc.subjectSheep
dc.subjectPCR
dc.subjectRFLP
dc.subjectDNA sequence analyses
dc.titleMolecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1
dc.typeArticle

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