Effect of captopril, an angiotensin-converting enzyme inhibitor, on morphine analgesia and tolerance in rats, and elucidating the inflammation and endoplasmic reticulum stress pathway in this effect
Date
2021Metadata
Show full item recordAbstract
The purpose of current study was to examine the possible involvement of captopril, an angiotensin-converting
enzyme inhibitor, on nociception, morphine analgesia and morphine tolerance development involving inflam mation and ER-stress pathways in rats. In this study, thirty-six male Wistar rats were used. Animals were divided
into six groups: Saline, 50 mg/kg captopril, 5 mg/kg morphine, morphine + captopril, morphine tolerance and
morphine tolerance + captopril. The resulting analgesic effect was measured with hot plate and tail flick
analgesia tests. The dorsal root ganglions (DRG) tissues were collected for inflammation parameters, endo plasmic reticulum (ER) stress and apoptosis proteins by using ELISA. Captopril showed anti-nociceptive effect
when given alone (p < 0.05 to p < 0.01). In addition, captopril increased the analgesic effect of morphine (p <
0.05 to p < 0.001) and also decreased the tolerance to morphine at a significant level (p < 0.05 to p < 0.001).
However, it decreased inflammation and ER-stress when applied with single-dose morphine and tolerance in duction (p < 0.001). Moreover, captopril decreased apoptosis proteins after tolerance development (p < 0.001).
In conclusion, captopril has antinociceptive properties, increasing analgesic effect of morphine, and preventing
tolerance development. These effects may occur by suppressing inflammation and ER-stress pathways.