Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization
Citation
To cite this article: Dursun Saraydın, H. Nursevin Öztop & Ceylan Hepokur (2021) Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization, Polymer-Plastics Technology and Materials, 60:1, 25-36, DOI: 10.1080/25740881.2020.1784223Abstract
Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68 degrees C and 30.44 degrees C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH-I and NSH-I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH-I showed excellent thermal, operational and pH stability.