dc.contributor.author | Gürkan, Ramazan | |
dc.date.accessioned | 2022-05-12T06:56:03Z | |
dc.date.available | 2022-05-12T06:56:03Z | |
dc.date.issued | 2021 | tr |
dc.identifier.citation | Q3 | tr |
dc.identifier.issn | 1060-3271 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/12862 | |
dc.description | Herhangi bir destek alınmamıştır. | tr |
dc.description.abstract | Background: Because of increasing amounts of 5-hydroxymethylfurfural (5-HMF) in processed foods and the complexity of the matrix, monitoring of trace 5-HMF requires accurate and reliable methods. Hence, an efficient sample pretreatment procedure is necessary for extraction and preconcentration of 5-HMF from the matrix.
Objective: In this study, a new and efficient sample preparation method utilizing ultrasound-assisted-cloud point extraction (UA-CPE), indirectly followed by flame atomic absorption spectrometry (FAAS), was introduced for the monitoring of trace amounts of 5-HMF in honey and jam samples.
Method: With this method, hydroxylamine was used as a derivatizing agent in the presence of Mn(II) and an anionic surfactant, SDS for extraction of 5-HMF at pH 9.0. For dispersing reagents in sample solution, low amounts of mixed surfactant, triton X-45 and SDS were mixed and fast-injected into the extraction media. A cloudy solution formed, and after reaction of 5-HMF with reagents, the cloudy solution was centrifuged. The extracted 5-HMF in the surfactant-rich phase was dissolved in acidic acetone and indirectly analyzed by FAAS.
Results: The method showed a detection limit of 1.27 lg/L in linear working range of 4–240 lg/L, good precision (2.3–6.5%), and recovery rates (93.5–97%) after preconcentration of 70-fold.
Conclusions: Within this study, an accurate and reliable method for the indirect quantification of 5-HMF in selected samples was successfully developed with a sensitivity improvement factor of 30.6.
Highlights: The figures of merit for the developed indirect method were appropriate. The applicability of the method for the analysis of 5-HMF in processed foods was excellent. | tr |
dc.description.sponsorship | - | tr |
dc.language.iso | eng | tr |
dc.publisher | OXFORD UNIV PRESS INCJOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 | tr |
dc.relation.isversionof | 10.1093/jaoacint/qsaa172 | tr |
dc.rights | info:eu-repo/semantics/openAccess | tr |
dc.subject | UA-CPE, 5-HMF, Mn(II), FAAS | tr |
dc.title | A New Enrichment Method for Quantification of 5-Hydroxymethylfurfural by Indirect Flame Atomic Absorption Spectrometry in Honey and Jam Samples | tr |
dc.type | article | tr |
dc.relation.journal | Journal of AOAC International | tr |
dc.contributor.department | Fen Fakültesi | tr |
dc.contributor.authorID | 0000-0003-0938-7697 | tr |
dc.identifier.volume | 104 | tr |
dc.identifier.issue | 3 | tr |
dc.identifier.endpage | 692 | tr |
dc.identifier.startpage | 682 | tr |
dc.relation.publicationcategory | Uluslararası Editör Denetimli Dergide Makale | tr |