Synthesis of PEGylated nanographene oxide as a nanocarrier for docetaxel drugs and anticancer activity on prostate cancer cell lines
Citation
Tas, A., & Keklikcioglu Cakmak, N. (2021). Synthesis of PEGylated nanographene oxide as a nanocarrier for docetaxel drugs and anticancer activity on prostate cancer cell lines. Human & Experimental Toxicology, 40(1), 172-182.Abstract
Graphene oxide (GO) has recently been considered one of the most promising carbon derivatives in nanotechnology. It has many excellent features such as tumor targeting ability, biocompatibility and low toxicity.
Therefore, we conjugated docetaxel (DTX) to GO-PEG molecule and investigate its anticancer efficacy in
prostate cancer cell line (DU-145). In order to obtain GO-PEG-DTX molecules, we conjugated the DTX via
bonds to PEG chains pegylated to the GO surface. We also investigated the stability of GO-PEG-DTX in
different biological fluids such as cell mediums, PBS and water in vitro conditions. GO-PEG-DTX has the
highest zeta potential in water. In the current research SEM, UV-Vis, and FTIR analyses and zeta potential were
utilized for the characterization of nano-sized GO-PEG-DTX. Anticancer efficacy of GO-PEG-DTX were then
investigated in DU-145 prostate cancer cell line using MTT metod. The prostate cancer cells were treated by
different concentrations of GO-PEG-DTX, GO-PEG, GO, and DTX (1–100 mg/ml) during 24, 48 and 72 h. The
spectrophotometric analyzed values at 570 nm were recorded and analysed with Graphpad Prism7. IC50
growth inhibition values was determined. The data showed that the GO-PEG-DTX had a highly effective
anticancer activity on prostate cancer cell lines after 24, 48 and 72 hours compared to other molecules.
GO-PEG-DTX was found statistically significant in the DU-145 cell line (***p < 0.0001, **p < 0.001, and
*p < 0.01). As a result, it can be said that PEGylated GO is an excellent nanocarrier system for the high
anticancer activity of DTX. Loading of anticancer drugs using this type of graphene-based nano carrier and
delivery to targeted tissues may find potential application in biomedicine.