Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors
Özet
Objectives: The aim of this study was to investigate BAP1,
OGT and YY1 genes and protein levels in 12 samples
(8 males, 4 females) of eyelid tumor tissue with basal cell
carcinoma (BCC) and 12 normal control subjects (8 males, 4
females).
Methods: The expression levels of these genes were
determined with RT-PCR and the protein levels and
expression using ELISA and IHC methods, respectively.
Results: In RT-PCR analysis, statistically significant
upregulated expression was determined of 1.84-fold of
BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05).
In the patient group, compared to the control group, there
was a similar statistically significant strong correlation
between the proteins (BAP1 and YY1; r = 0.850, BAP1 and
OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the
ELISA and IHC analysis methods, a significant increase in
BAP1 and YY1 protein expression levels was observed
compared to the control group (p < 0.05).
Conclusions: The study results demonstrated that BAP1
and YY1 genes and protein levels were upregulated in
eyelid tumor tissue with BCC.