Antimicrobial and antibiofilm activities and bovine serum albumin binding properties of benzimidazolium derivative NHC salts and their Ag(I)-NHC complexes
Özet
Benzimidazole type N-heterocyclic carbene molecules and their metal complexes
have well-known biological activity. Elemental silver and silver complexes
also have antibacterial and antifungal activity. In this study, silver
complexes with 1-allyl 5,6-dimethylbenzimidazolium derivative ligands were
analyzed for their antimicrobial and antibiofilm activities. Additionally, bovine
serum albumin (BSA) binding properties of the molecules were analyzed by
spectrophotometric methods, and the details of interactions of the molecules
with BSA were investigated by molecular docking methods. While
N-heterocyclic carbene salts showed moderate antifungal and antibiofilm
activity, silver complexes of these salts have strong antimicrobial activity with
<1.9–15.6 μg/ml minimal inhibitory concentration (MIC) values. On the other
hand, carbene salts have better affinity against BSA than the complexes. Additionally,
the interaction of the optimized complexes with SarA and BSA were
analyzed by molecular docking methods. According to in vitro and in silico
evaluation, the studied Ag complexes may be utilized for advanced studies.