Molecularly imprinted composite discs for transferrin recognition
Citation
Gülgün Aylaz, Okan Zenger, Gözde Baydemir Peşint & Müge AndaçAbstract
Human Transferrin (HTr) imprinted composite cryogel (HTr-MIPCC) discs were synthesized with
distinctive structure and increased adsorption capacity and specificity for HTr by combining the
advantages of the surface imprinting technique. HEMA-based cryogel was embedded with HTrsurface
imprinted particles. Thanks to the imprinted particles embedding, both the higher surface
area and specific recognition are provided. The structure of the HTr-MIPCC was characterized by
FTIR Spectrometer, SEM, swelling studies, flow dynamics, and surface area measurements. The
addition of surface imprinted particles into the cryogel disc has resulted in an increased surface
area, enhancing the capacity of composite cryogel for HTr adsorption up to 10.96 mg/g and gel
fractionation yields reached up to 83%. Selectivity studies were carried out against HIgG and HSA as
competitors. The HTr-MIPCC discs were packaged into an FPLC column for selective depletion of
HTr. It was calculated that the HTr-MIPCC is 1.071 and 1.062 times selective for HTr than HSA and
HIgG, respectively. According to FPLC studies HTr-MIPCC can be considered as an excellent alternative
for affinity matrixes used for the detection and selective recognition of HTr directly from
human serum. HTr-MIPCC discs can be used several times without any noteworthy reduction in the
adsorption capacity of HTr