Biological effects and molecular docking studies of Catechin 5-O-gallate: antioxidant, anticholinergics, antiepileptic and antidiabetic potentials

Abstract

Gallocatechin gallate is a form of catechin and an ester of gallocatechin and gallic acid. This is an epimer of the gallate epigallocatechin. In this study, the effect of this molecule, containing a biologically active group, was investigated in terms of important metabolic enzymes (carbonic anhydrase isoenzymes I and II (hCA I and II), achethylcholinesterase (AChE) and a-glycosidase (a-Gly) enzymes). The molecular docking method used to compare the biological activities of the Catechin 5-O-gallate molecule against enzymes was used. Afterwards, the ADME/T analysis was performed to investigate the drug availability of the Catechin 5-O-gallate molecule and the parameters obtained from ADME/T analysis were examined. Continuation of this study, for evaluating antioxidant and radical scavenging capacity Catechin 5-O-gallate, cupric ion (Cu2þ) reduction capacity by CUPRAC method, Fe3þ-Fe2þ reducing capacity, DPPH free radical clarifying (DPPH· ), ABTS radical clarifying (ABTS þ) were performed separately and during the study, trolox, a-tocopherol BHT and BHA were used as the reference antioxidant compound. Comparisons were applied with the four standard substances. Abbreviations: ADME: absorption, distribution, metabolism and excretion; ACR: acarbose; AZA: acetazolamide; AChE: achethylcholinesterase; ABTS: 2, 2’-azino-Bis-3-ethylbenzothiazoline-6-sulfonic acid (biochemical reagent); a-Gly: a-glycosidase; BHA: butylated hydroxyanisole; BHT: butylated hydroxytoluene; BChE: butyrylcholinesterase; CA: carbonic anhydrase; CUPRAC: cupric reducing antioxidant capacity; DPPH: 2,2-diphenyl-1-picrylhydrazyl; EGCG: gallate epigallocatechin; GA: gallic acid; GCG: gallocatechin gallate