THE CYTOTOXIC EFFECTIVENESS OF PROPOLIS SAMPLES FROM SIVAS PROVINCE ON SK-OV-3 CELL LINE

Abstract

Honey, pollen, propolis and other bee products are consumed as food from ancient times and are also natural products that are used as a traditional medicine in the treatment of many diseases [1-5]. The widespread use of all bee products to strengthen the immune system is a very popular topic in recent times. Today, the use of bee products in medical applications, as a food supplement, beverage substances, as a source of antioxidant, and antiaging in the cosmetic industries are becoming extremely common. Studies in the literature have shown that there are significant differences between the contents and biological activities of bee propolis from different botanical origins and different regions [6]. Within the scope of this study, propolis samples were obtained from various localities of Sivas, where beekeeping is intense. Propolis samples were obtained from beekeepers in the 2021 harvest period, and extractions were carried out in the laboratory using ethyl alcohol and water solvents. The scientific studies on bee products of Sivas province are very limited. In this study, it was aimed to determine and compare the cytotoxic activity of propolis samples collected different area of Sivas province in SK-OV-3 (Human ovarian cancer) cell line using MTT cell proliferation kit. MTT results were determined spectrophotometrically and IC50 values were calculated with the help of numerical data obtained by GraphPad Prism Version 8.4.1 software program. It was observed that ethyl alcohol isolates of propolis samples showed good antiproliferative activity in SK-OV-3 cell line (IC50 < 100 μg/mL). It is the first time to test the efficacy of propolis samples from Sivas region on the SKOV-3 cell line. It is thought that this study will be a beginning in terms of the therapeutic use of bee propolis samples from Sivas province and the preparation of new effective supplements