dc.contributor.author | Şahin Bilal | |
dc.contributor.author | Özdemir Ercan | |
dc.contributor.author | Gümüş Erkan | |
dc.contributor.author | Ergül Mustafa | |
dc.contributor.author | Taşkıran Ahmet Şevki | |
dc.date.accessioned | 2023-06-23T09:43:03Z | |
dc.date.available | 2023-06-23T09:43:03Z | |
dc.date.issued | 2022 | tr |
dc.identifier.citation | ahin, B., Ozdemir, E., Gumus, E., Ergul, M., & Taskiran, A. S. (2022). The 5-HT7 receptor antagonist SB-269970 alleviates seizure activity and downregulates hippocampal c-Fos expression in pentylenetetrazole-induced kindled rats. Neurological research, 44(9), 786–796. https://doi.org/10.1080/01616412.2022.2064700 | tr |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/14079 | |
dc.description.abstract | Objectives: Recently, studies have demonstrated that serotonin type 7 receptors (5-HT7) have conflincting effects on neuronal excitability in different brain regions. However, the effect of 5-HT7 on seizures has not been exactly elucidated yet. Therefore, our aim in this study was to investigate the effects of 5-HT7 antagonist SB-269970 on pentylenetetrazole (PTZ) induced fully kindled rats.
Methods: In the study, 32 adult male Wistar Albino rats (weighing 220-260 g) were used. Rats were injected with PTZ (35 mg/kg) intraperitoneally every other day to generate kindling model. 5-CT (0.1 mg/kg) and SB-269970 (1 mg/kg) were administered 30 min before acute seizure induction with PTZ (35 mg/kg). Seizure stages were determined according to the Racine scale. After electrocorticography (ECoG) recordings of seizure-induced rats were obtained, the animals were sacrificed by decapitation. The hippocampal GABA levels were determined by ELISA kit and the number of c-Fos positive neurons in the hippocampal dentate gyrus (DG), CA1 and CA3 areas were measured by immunohistochemical method.
Results: The results showed that SB-269970 reduced the number of spikes, percent seizure duration and duration of generalized tonic-clonic seizures (dGTCS), while increasing the onset time of generalized tonic-clonic seizures (oGTCS). The hippocampal GABA levels were significantly increased in the SB-269970 group compared with the PTZ group. In addition, SB-269970 reduced the number of c-Fos positive cells in hippocampal CA1 area.
Discussion: 5-HT7 antagonist SB-269970 displays anticonvulsant effects on PTZ-induced seizures in fully kindled rats and these effects may be related to GABAergic activity in the hippocampus. | tr |
dc.language.iso | eng | tr |
dc.publisher | NEUROLOGICAL RESEARCH | tr |
dc.relation.isversionof | 10.1080/01616412.2022.2064700 | tr |
dc.rights | info:eu-repo/semantics/openAccess | tr |
dc.subject | 5-HT receptor; 5-HT7; GABA; SB-269970; c-Fos; seizure | tr |
dc.title | The 5-HT7 receptor antagonist SB-269970 alleviates seizure activity and downregulates hippocampal c-Fos expression in pentylenetetrazole-induced kindled rats | tr |
dc.type | article | tr |
dc.contributor.department | Tıp Fakültesi | tr |
dc.contributor.authorID | 0000-0002-4419-1385 | tr |
dc.identifier.volume | 44 | tr |
dc.identifier.issue | 9 | tr |
dc.identifier.endpage | 796 | tr |
dc.identifier.startpage | 786 | tr |
dc.relation.publicationcategory | Ulusal Hakemli Dergide Makale - Kurum Öğretim Elemanı | tr |