First molecular detection and phylogenetic analysis of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos in cattle in different parts of Kyrgyzstan

Abstract

Hemoplasma species cause clinical infections ranging from asymptomatic to life-threatening in cattle. To date, two hemoplasma species, Myocplasma wenyonii (M. wenyonii) and Candidatus Mycoplasma haemobos (Ca. M. haemobos), have been identified from cattle. There is no data on the presence and distributions of bovine hemoplasma species in cattle in Kyrgyzstan, notwithstanding their potential negative economic impact on cattle production industries. In the current study, we investigated the molecular presence and distributions of bovine hemoplasma species from 358 cattle samples obtained from different parts (specifically in the Chuy, Talas, Djalal-Abad, Naryn, and Issyk-Kul regions) of Kyrgyzstan. Bovine hemoplasma species were found in 75 of 358 (20.94%) animals. Of these 358 samples, 42 (11.73%) were only positive for M. wenyonii and 21 (5.86%) only for Ca. M. haemobos, whereas 12 (3.35%) cattle were infected with both species. Randomly selected four positive (two samples for each species) Polymerase Chain Reaction (PCR) products were sequenced, and the consensus sequences were uploaded to GenBank. The nucleotide similarity rates were found to be 97.33-100% for M. wenyonii and 99.31-100% for Ca. M. haemobos when compared with M. wenyonii and Ca. M. haemobos sequences present in the GenBank, which were uploaded from different countries. This study provided data on the molecular presence and phylogenetic analysis of M. wenyonii and Ca. M. haemobos for the first time in cattle in Kyrgyzstan. Thus, farmers and veterinarians should take precautions against bovine hemoplasma species both to protect animals and to increase the profitability of the farms in Kyrgyzstan.