| dc.description.abstract | Idiopathic pulmonary fibrosis (IPF) is a serious progressive pulmonary disease of
unknown etiology and high mortality. Carvacrol is a natural phenolic monoterpene with various
pharmacological effects, especially antioxidant and anti inflammatory effects. Hence, the present
study aimed to investigate the effect of carvacrol on bleomycin (BLM) induced pulmonary fibrosis
(PF) in Wistar albino rats. Rats were administered a single dose of BLM (5mg/kg, intratracheal) or
vehicle and treated with carvacrol (100 mg/kg, p.o. for 14 days following BLM administration). For
calculating the lung index, the body and lungs were weighed. The Elisa method was used to assess
hydroxyproline content, anti inflammatory, and antioxidant effects. Fibrosis score, collagen
deposition and inflammation were evaluated with Hematoxylin Eosin (HxE) and Masson’s
trichrome staining. Inducible nitric oxide synthase (iNOS), transforming growth factor beta 1
(TGF β1), and caspase 3 expressions were assessed immunohistochemically. BLM administration
significantly diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities
and increased malondialdehyde (MDA) levels. BLM also increased tumor necrosis factor alpha
(TNF α) and collagen bundle accumulation. Carvacrol at 100 mg/kg significantly decreased
collagen accumulation, MDA, TNF α levels, iNOS, TGF 1, and caspase 3 expression, while
increasing SOD and GPx activity. Histopathological examination supported the findings that
carvacrol attenuated the degree of collagen deposition and inflammation. This study revealed that
treatment with carvacrol (100 mg/kg) exhibits a potential healing effect on BLM induced PF by
reducing inflammatory and oxidative damages and histopathological alterations, with possible
molecular targets being iNOS, TGF β1 and caspase 3 signaling pathways. | tr |
