| dc.description.abstract | The immobilization of lysozyme onto a novel
synthetic metal chelator composite based on chitosan/
PAA-PMA (chitosan/acrylamide-maleic acid) and its effect
on antimicrobial activity were the aim of the current
study. The plain composite and the lysozyme immobilized
composite were characterized according to scanning
electron microscopy (SEM), Ultraviolet spectroscopy (UV),
Fourier transform infrared (FT-IR), and X-ray diffraction
(XRD) analysis. Furthermore, the activities of lysozyme and
immobilized lysozyme were investigated, as well as their
antimicrobial activity against Escherichia coli, Klebsiella
pneumoniae, Pseudomonas aeruginosa, Enterococcus
faecalis, and Staphylococcus aureus, as well as their
cytotoxic effects, which were both approximately greater
than those of free lysozyme. The last step was cytotoxic
studies on lysozyme, chitosan composite, and lysozymeimmobilized
chitosan composite, which showed no
cytotoxic effect. Several investigations have shown that
metal chelators improve lysozyme’s antibacterial action.
Two carboxylic acids containing maleic acid were used as a
metal chelator in this study. Lysozyme was immobilized
from a side other than carboxylic acids, so that carboxylic
acid groups, which are metal chelating groups, do not
prevent synergy by competing with metal binding at
neutral pH. This enhanced antimicrobial activity. | tr |
