The effect of hesperidin added to quail diets on blood gas, serum biochemistry and Hsp70 in heat stress

Abstract

The aim of this study was to determine the effects of flavonoid, which is a product ofcitrus production, on blood parameters and HSP 70 concentration in quails applied atthermoneutral and heat stress. In this study, 160 quails (Coturnix coturnix japonica,male), 6 wk old and 150-200 g live weight, were housed in cages for 1 wk of exercise and5 wk of trial period. The study design consists of 4 groups of 40 animals and 4 subgroupswith 10 animals in each group. Thermoneutral (24 ± 0.1 o C) groups are NC (0 ghesperidin/kg basal feed) and NHES3 (3 g hesperidin /kg basal feed) and heat stress (34± 0.1 oC) groups are HC (0 g hesperidin/kg basal feed) and HHES3 (3 g hesperidin /kgbasal feed) were randomly generated. In the case of heat stress, pO2, pH, HCO3, Clconcentrations decreased in the HHES3 group compared to the HC group (P<0.05). ALPenzyme concentration showed a significant decrease in the HHES3 group compared tothe HC group in the heat stress condition. Heat shock protein (HSP70) protein levelincreased in blood serum, kidney, liver and thigh tissues in HC group with cellular stressduring heat stress; however, HSP70 concentration decreased significantly in the HHES3 group. As a result, positive effects of hesperidin supplementation in the diet were foundin both heat stress and thermoneutral conditions.