Effect of hesperidin supplementation on blood profile, antioxidant capacity, intestinal histomorphology and fecal microbial counts in Japanese quails

Abstract

This study was conducted to determine the effects of hesperidin, a flavonoid added toquail diets, on blood serum, enzymes in tissues, intestinal histomorphology and fecalmicroflora. In the study, first treatment [(control) (0g hesperidin/kg feed)], secondtreatment [(HES1) (1 g hesperidin/kg feed)], third treatment [(HES2) (2 g hesperidin/kgfeed)] was added to with the basal diet through 35 d. The study was carried out with 3main groups, 20 quails with 5 sub-repeats in each group and a total of 300 quails. At theend of the study, blood, liver and thigh muscle tissue and fecal samples were taken.Alanine transaminase (ALT) and lactate dehydrogenase (LDH) decreased in the HES1group but increased in the HES2 group compared to the control group (P<0.05). Aspartatetransaminase (AST) increased in the HES1 group compared to the control group and decreased in the HES2 group (P<0.05). Amylase, on the other hand, showed a regularincrease in HES1 and HES2 groups to which hesperidin was added to the control groupin hesperidin added groups (P<0.05). The tissue antioxidant GSH, CAT and SOD enzymeparameters showed a significant increase in the hesperidin added groups compared to thecontrol group, and this increase was found to be significant compared to the control group(P<0.05). In intestinal histomorphology, in hesperidin treatment groups increased theheight of villus in the cecum tissue; in colon tissue, it was determined that hesperidinadded groups increased villus height but decreased crypt depth (P<0.05). Consequently,diets hesperidin with treatments positively is thought to affect the lipid, thigh, liver andserum antioxidant enzyme levels, intestinal histomorphology and fecal microflora inquail.