Investigation of the effect of sugammadex on glutamateinduced neurotoxicity in C6 cell line and the roles played by nitric oxide and oxidative stress pathways
Date
August 202Metadata
Show full item recordAbstract
This experiment was intended to evaluate the effect of sugammadex on the
cytotoxicity induced by glutamate, involving the nitric oxide and oxidative
stress pathways. C6 glioma cells were used in the study. Glutamate was
given to cells in the glutamate group for 24 h. Sugammadex at different
concentrations was given to cells in the sugammadex group for 24 h. Cells
in the sugammadex + glutamate group were pre-treated with sugammadex
at various concentrations for 1 h and then exposed to glutamate for 24 h.
XTT assay was used to assess cell viability. Levels of nitric oxide (NO),
neuronal nitric oxide synthase (nNOS), total antioxidant (TAS), and total
oxidant (TOS) in the cells were calculated using commercial kits. Apoptosis
was detected by TUNEL assay. Sugammadex at concentrations of 50 and
100 μg/mL significantly enhanced the cell viability in C6 cells after the
cytotoxicity induced by glutamate (p < 0.001). Moreover, sugammadex
considerably decreased the levels of nNOS NO and TOS and the number
of apoptotic cells and increased the level of TAS (p < 0.001). Sugammadex
has protective and antioxidant properties on cytotoxicity and could be an
effective supplement for neurodegenerative diseases such as Alzheimer
and Parkinson if further research in vivo supports this claim.