dc.contributor.author | Murat Doğan | |
dc.contributor.author | Ümit M Koçyiğit | |
dc.contributor.author | Feyza Nur Çetin | |
dc.contributor.author | Beyza Nur Yılmaz | |
dc.contributor.author | Turgut Taşkın | |
dc.date.accessioned | 2024-03-07T07:54:56Z | |
dc.date.available | 2024-03-07T07:54:56Z | |
dc.date.issued | 2023/1/9 | tr |
dc.identifier.citation | APA | tr |
dc.identifier.uri | https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bab.2437 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12418/14851 | |
dc.description | . | tr |
dc.description.abstract | Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High-performance liquid chromatography diode array detector (HPLC-DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8-hydroxy salvigenin component. | tr |
dc.description.sponsorship | Sivas Cumhuriyet University Scientific
Research Project Fund, Grant/Award
Number: ECZ-2022-085 | tr |
dc.language.iso | eng | tr |
dc.publisher | JUMB | tr |
dc.relation.isversionof | https://doi.org/10.1002/bab.2437 | tr |
dc.rights | info:eu-repo/semantics/closedAccess | tr |
dc.subject | Carbonic anhydrase isoenzyme I, Carbonic anhydrase isoenzyme II | tr |
dc.title | Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles | tr |
dc.title.alternative | . | tr |
dc.type | article | tr |
dc.relation.journal | Biotechnology and Applied Biochemistry | tr |
dc.contributor.department | Eczacılık Fakültesi | tr |
dc.contributor.authorID | https://orcid.org/0000-0001-8710-2912 | tr |
dc.identifier.volume | 70 | tr |
dc.identifier.issue | 3 | tr |
dc.identifier.endpage | 1278 | tr |
dc.identifier.startpage | 1270 | tr |
dc.relation.publicationcategory | Uluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanı | tr |