Propolis nanoparticles synthesis and characterization with cytotoxic and apoptotic effects on breast cancer cells
Date
2023Author
Ay,Ebru NurCaner,Armağan
Hepokur,Ceylan
Danişman Kalindemirtaş,Ferdane
Eroğlu, Güneş Özen
Kariper,Afşin
Metadata
Show full item recordAbstract
The aim of this work was to investigate the antiproliferative and pro-apoptotic effects of propolis
on human breast cancer cells using a simple and inexpensive nanoformulation. Various meth ods were used to characterize the surface and morphology of propolis nanoparticles (NP-Pro),
including UV-VIS, FTIR, DLS, and EDX-SEM. The cytotoxic activity of MCF-7, MDA-MB-231 breast
cancer cells was measured by XTT technique and compared with healthy cells (MCF-10A). The
apoptotic effect of NP-Pro was assessed by flow cytometry using Annexin/PI. The IC50 values of
NP-Pro on MCF-7, MDA-MB-231 and MCF-10A cells were 13.67 ± 0.89, 17.89 ± 0.6, 29.9 ± 0.56 μg,
respectively. According to these findings, the NP-Pro form we prepared was more cytotoxic on
cancer cells than propolis. Cancer cells had much stronger apoptotic activity than healthy cells
(p < 0.0001). In this work, NP-Pro was produced through a distinct, straightforward, and low-cost
method. In summary, the NP-Pro we synthesized may be a promising potential agent for breast
cancer treatment due to its specific cytotoxicity via apoptosis.