Chemical and functional composition and biological activities of Anatolian Hypericum scabrum L. plant

Abstract

Hypericum scabrum L. plant has wide use in ethnopharmacology and is cultivated both naturally and technologically. This study was conducted to determine chemical and functional properties and in vitro biological activities as antimicrobial, antioxidant, anticancer composition of H. scabrum L. (Hypericaceae) from Anatolia. This plant was collected from Elazıg and Sivas province of Turkey. The ICP-OES method was used for the investigation of the heavy metal composition and GC–MS analysis was used for the defining of chemical components. Antimicrobial qualifications of plant extracts were investigated on 8 different microorganisms by minimum inhibition concentration (MIC) method. Antioxidant activities were inves- tigated by using Diagnostic kits, which provide 99% reliable results with chemical reduction reactions. In vitro anticancer properties of the extracts were investigated on 5 different cell lines by using MTT method. It was observed that the flower and leaf parts of Hypericum plant have a very high antioxidant capacity and has mediocre antimicrobial activity only on 2 microorganisms. Also moderate cytotoxic ac- tivities on some cell lines were determined. It is thought that H. scabrum plant has functional bioactive compounds and might be an