Sıçanlarda Karaciğer İskemi Reperfüzyon Hasarında Piknogenol®'ün İyileştirici Etkileri

Abstract

Amaç: Piknogenol® (PYC®), Pinus maritima bitkisinin kabuğundan elde edilen ve birkaç tip fenolik içerikten oluşan, suda çözünür standardize bir Bulgular: TOS, TTL, MPO, AOPP, ALT, AST and LDH düzeyleri IR+PYC® grubunda IR grubuna göre anlamlı ölçüde düşük bulundu (p<0.05). TAS düzeyi IR+PYC® grubunda IR grubuna göre anlamlı ölçüde yüksek bulundu (p<0.05). DNA hasarı değerlendirildiğinde PYC® verilen grupta DNA iskemi grubuna göre anlamlı ölçüde azaldığı saptandı (p<0.05).Sonuç: Mevcut bulgular, piknogenol tedavisinin IR ile indüklenmiş oksidatif hasarı DNA hasarını azaltarak ve antioksidan durumu arttırarak da önlediğini göstermektedir.

Pycnogenol&reg; (PYC&reg;), a standardized extract from the bark of Pinus maritima, consists of different phenolic compounds. PYC&reg; has shown to have protective effects on chronic diseases such as diabetes, asthma, cancer, and immune disorders. The aim of this study was to determine the effects of PYC&reg; against the DNA damage and biochemical changes in blood, liver, and lung tissues of ischemia-reperfusion (IR)-induced Wistar albino rats. Materials and Methods: A sham group, IR injury-induced group, and IR+PYC&reg; group were formed. Ischemia was induced and sustained for 45 min, then the ischemic liver was reperfused, which was sustained for a further 120 min at the end of this period. After anesthesia and before the IR inducement, 100 mg/kg PYC&reg; was given to the IR+PYC&reg; group through intraperitoneal injections. The total oxidant (TOS) and total antioxidant status (TAS), total thiol levels (TTL), advanced oxidation protein products (AOPP), and biochemical parameters [myeloperoxidase (MPO), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH)] in the rats were analyzed using spectrophotometric methods and DNA damage was assessed using single-cell gel electrophoresis. Results: The levels of TOS, TTL, MPO, AOPP, ALT, AST, and LDH were significantly decreased in the IR+PYC&reg; group compared with the IR group (p&lt;0.05). The levels of TAS were significantly increased in the IR+PYC&reg; group compared with the IR group (p&lt;0.05). PYC&reg; reduced the DNA damage when compared with the IR group (p&lt;0.05). Conclusion: The present results suggest that PYC&reg; treatment might have a role in the prevention of IR-induced oxidative damage by decreasing DNA damage and increasing antioxidant status.