Green synthesis, characterization, anti-bacterial and cytotoxic effects of zinc oxide nanoparticles using aqueous extract of artichoke leafs [Enginar yaprağı sulu ekstraktı kullanılarak çinko oksit nanopartiküllerinin yeşil sentezi, karakterizasyonu, anti-bakteriyel ve sitotoksik etkileri]
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Aim: The aim of this study is to synthesize zinc oxide nanoparticles (ZnONPs) by green chemistry method and investigate anti-bacterial and anticancer effects of these nanoparticles. Material and Methods: ZnONPs were synthesized by the green chemistry method using zinc ions and aqueous artichoke leaf (Cynara scolymus) extract. Ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), zetasizer and energy dispersive X-ray spectroscopy (EDX) were used to confirm the formation and characterization of ZnONPs. Antibacterial activities of ZnONPs on four different bacteria species (E. coli, S. aureus, P. aeruginosa and E. faecalis) were measured by minimal inhibitory concentration (MIC) and agar well diffusion method. Cytotoxic effects of ZnONPs on HT-29 human colon cancer cells were determined as concentration and time dependent. Results: In the UV-Vis spectrum, absorbance increase was observed in 320-335 nm range which is specific to ZnO. In the FTIR spectrum, stretching vibrations of ZnO were determined in 426 cm-1 and 540 cm-1. The particle size was 276-309 nm in SEM analysis. In the zeta-sizer measurements of ZnONPs, the particle size was 137.8 nm and the particle charge was-6.34 meV. In the antibacterial activity measurements, it was determined that synthesized nanoparticles induce inhibition of bacterial activity in E. coli and S. aureus. ZnONPs showed cytotoxic effects on HT-29 cells at concentrations higher than 10 µg/mL. Conclusion: This study showed that ZnONPs can be prepared at low cost and have potential to be used as a carrier system for new drug formulations in clinical therapies. © 2019, Duzce University Medical School. All rights reserved.