Yazar "Kumru, Alper Serhat" seçeneğine göre listele
Listeleniyor 1 - 11 / 11
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECT OF ALLIUM SCORODOPRASUM L. ETHANOLIC EXTRACT (ASE) IN MICE(JAPS, October, 2022) Ekici, Mehmet; Güngör, Hüseyin; Demir, Tuğba; Ateş, Mehmet Burak; Kumru, Alper SerhatThe antioxidant and anti-inflammatory properties of Allium species plants have been reported in studies. However, no study was found showing the antioxidant and anti-inflammatory properties of Allium scorodoprasum L. ethanolic extract (ASE) in the experimental carrageenan (Carr) induced paw edema mice model. This study aimed to investigate the antioxidant and anti-inflammatory effects of ASE in Carr-induced paw edema mice model. The polyphenolic compounds of ASE were analyzed with HPLC. A total of 30 male Balb/C mice were randomly divided into Control, Carr, Carr + Diclofenac Sodium, Carr + ASE200, Carr + ASE400, and Carr + ASE600 groups (n=5/group). The mice paw edema was induced by 0.05 ml subplantar injection of 1% Carr. Different groups of mice with Carr-induced paw edema were administered by oral gavage with 10 mg/kg Diclofenac Sodium and 200, 400, and 600 mg/kg ASE, respectively. Orally administered ASE at 400 and 600 mg/kg significantly decreased paw swelling, and serum levels of TNF-α, IL-1β, IL-6, AST, ALT, and ALP. ASE also decreased MDA levels and total oxidant status (TOS) but increased total antioxidant status (TAS) in the liver. In addition, ASE decreased paw and liver histopathologic values and neutrophil counts in paw tissues. The results of this study showed that ASE has an anti-inflammatory and antioxidant effect through strong polyphenolic compounds.Öğe Effect of Astragalus microcephalus wild extract and Vitamin E–Selenium combination on Cadmium–induced damage in rat ovaries(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Kurt, Begum; Kara, Haki; Sahın, Mahmut; Kumru, Alper Serhat; Ozkaraca, MustafaThis study was conducted to investigate the protective effect of Astragalus (AST) extract alone and in combination with vitamin E (vit E) + selenium (Se) against the toxicity induced by cadmium (CdCl2) in rat ovaries. Thirty–six female Wistar rats were divided into six groups. AST was administered at a dose of 5 mg·kg-1, Cd at a dose of 2 mg·kg-1, and Vit E (60 mg·kg-1) + Se (1 mg·kg-1) orally for a duration of 15 days. The levels of MDA, GSH–Px, SOD, and CAT were analyzed in the blood and tissue samples to assess oxidative stress. Additionally, the levels of estrogen, FSH, LH, Inhibin B, and Antimullerian hormones were measured in the serum samples. The ovarian tissues were examined histopathologically and immunohistochemically for 8–OhDG, Caspase 3, and LC3B immunoreactivity. In the group exposed to CdCl2, MDA levels significantly increased, while antioxidant parameters showed significant decreases (P<0.05). Although significant improvements were observed in the groups treated with AST alone, more significant improvements were seen in the groups treated with both AST and Vit E + Se (P<0.05). It was concluded that AST extracts alone and in combination with Vit E + Se exhibited protective effects against ovarian toxicity caused by Cd exposure and may be effective against metal toxicity. © (2024), (Universidad del Zulia, Facultad de Ciencias Veterinarias). All rights reserved.Öğe Effects of apixaban, rivaroxaban, dabigatran and enoxaparin on histopathology and laboratory parameters in Achilles tendon injury: An in vivo study(Wolters Kluwer Medknow Publications, 2021) Avci, Sema; Gungor, Huseyin; Kumru, Alper Serhat; Sahin, Mahmut; Gezer, Arzu; Gok, Uzeyir; Kara, HakiObjectives: To compare the effects of apixaban, rivaroxaban, dabigatran and enoxaparin on histopathology and blood parameters in rats with Achilles tendon injury. Materials and Methods: Thirty adult, male Wistar albino rats weighting 220-240 g were randomly divided into five (one control and four treatment) groups and placed in a controlled environment. The Achilles tendon was incised and re-sutured in each rat, after which each group was provided the following treatment for 28 days: a) 2 ml saline to the control group, b) apixaban in 1 ml of saline (10 mg/kg/day) +1 ml of saline, c) rivaroxaban in 1 ml of saline (2 mg/kg/day) +1 ml saline, d) dabigatran in 1 ml of saline (30 mg/kg/day) +1 ml of saline, e) enoxaparin (80 mu g/kg/day) + 2 ml of saline. Results: Hemogram, biochemical and coagulation parameters differed significantly between the control and treatment groups (P < 0.05). Compared with the control group, in the apixaban group, type I and type III collagen immunoreactivity were severe and moderate, respectively. In the rivaroxaban and dabigatran groups, both type I and type III collagen immunoreactivity were medium and severe, respectively. In the enoxaparin group, type I and type III collagen immunoreactivity were mild and severe, respectively. Conclusion: The higher concentration of type I collagen in the apixaban and dabigatran indicates faster tendon healing in these groups, and the higher concentration of the type III collagen in the enoxaparin group indicates slower healing in this group.Öğe Endemik Astragalus microcephalus Wild Bitkisi Ekstraktının Ratlarda Deneysel Oluşturulan Yara Üzerine Etkisinin Araştırılması(2023) Şahin, Mahmut; Kumru, Alper Serhat; Özkaraca, Mustafa; Yılmaz, Gökhan; Kara, HakiÖZ Amaç: Bu çalışmanın amacı endemik Astragalus microcephalus wild (AST) bitkisi ekstraktının ratlarda deneysel oluşturulan yara üzerine etkisinin belirlenmesidir. Gereç ve Yöntem: Astragalus microcephalus. wild (AST) örneklerinden elde edilen ekstrakt, lanolin kullanılarak %2.5 ve %5’lik AST pomadı haline getirildi. Yine, ticari dekspantenol (Deks) (dekspantenol 500 mg/2ml, Bepanthen®) kullanılarak %2.5 ve %5’lik pomadlar oluşturuldu. Çalışmada her grupta 4 hayvan yer aldı.15 gün boyunca kontrol grubu hariç tüm hayvanlara pomad uygulaması günde bir kez yapıldı. Çalışmanın 3., 6., 9., 12. ve 15. günlerinde yara boyutları ölçüldü. 15. Günün sonunda dekapite edilerek yara bölgeleri histopatolojik inceleme için alındı. Bulgular: Epitelizasyon şekillenmesi, Mononükleer hücre infiltrasyonu ve granülasyon dokusu açısından gruplar arasında önemli farklılıklar (p <0.05) olduğu belirlendi. AST %2.5 ve AST 5 gruplarında ise epitel oluşumunun devam ettiği ve keratinleşmenin orta düzeylere yükseldiği belirlendi. Mn hücre infiltrasyonlarının Ast %5 grubunda hafiflediği görüldü. Granulasyon doku oluşumunun Dex %5, AST %2.5 ve AST %5 gruplarında olgunlaşmanın artmasıyla orta seviyede olduğu tespit edildi. Sonuç: Astragalus microcephalus wild ekstraktının yara iyileşmesi üzerine etkilerinin belirlenmesi amacıyla hazırlanan %2.5 ve % 5’lik pomatlarının yara iyileşmesini hızlı bir şekilde sağladığı görülmüştür.Öğe Investigation of the Protective Effects of Dexpanthenol and Boldine in Cisplatin-Induced Experimental Testis Injury Model in Rats(2022) Tuna, Zeynep; Kara, Haki; Şahin, Mahmut; Güngör, Hüseyin; Kumru, Alper SerhatThis study was carried out to determine the protective effects of dexpanthenol and boldin against \rpossible testicular damage caused by cisplatin. 48 Male Wistar rats (250-270 g) aged 4-5 months \rwere used in the study. 8 groups were formed with 6 animals in each group. Boldin (20 and 40\rmg/kg) and dexpentanol (250 and 500 mg/kg) were administered to rats along with cisplatin (7 \rmg/kg). At the end of the 14th day, spermatogenic and histopathological examinations and \rtesticular tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase \r(GSH-Px) and catalase (CAT) levels were determined. A significant decrease in sperm quality and \rmotility, a significant increase in MDA, and a significant decrease in SOD, GSH-Px, and CAT levels \rwere observed in the cisplatin group. MDA levels were significantly lower in the other groups \rcompared to the cisplatin group. SOD, GSH-Px and CAT enzyme levels increased in the treatment \rgroups compared to the cisplatin group and approached the control group. Combined application of \rhigh doses of both substances with cisplatin reduced histopathological lesions. Significant \rincreases were found in the groups in which both doses of dexpanthenol and boldin were \rcombined with cisplatin compared to the cisplatin group. As a result, it was concluded that \rdexpanthenol and boldin treatments may have a protective effect against cisplatin-induced \rtesticular damage in rats.\rÖğe Koyunlarda Enrofloksasinin Farklı Dozlarda Tekrarlı Uygulamasının Hematolojik ve Biyokimyasal Parametrelere Etkisi(2021) Güngör, Hüseyin; Kumru, Alper Serhat; Yılmaz, Gökhan; Coskun, AlparslanAmaç: Bu araştırmanın amacı sağlıklı koyunlarda enrofloksasinin farklı dozlardaki (2.5-5 mg/kg) tekrarlayan (günde bir defa, 5 gün) damar içi uygulamasının hematolojik ve biyokimyasal parametrelere etkisini belirlemektir. Gereç ve Yöntem: Araştırmada Akkaraman ırkı 12 adet dişi koyun kullanıldı. Hayvanlar her grupta 6 adet olacak şekilde iki gruba ayrıldı. Enrofloksasin gruplardan birisine 2.5 mg/kg dozda diğerine ise 5 mg/kg dozda damar içi (v. jugularis) yolla günde bir defa 5 gün boyunca uygulandı. Kan örnekleri 0. (Kontrol) ve 120. saatlerde alındı. Hematolojik parametreler (WBC, RBC, HGB, HCT ve PLT) kan hücre sayım cihazında ve biyokimyasal parametreler (AST, ALT, ALP, GGT, kolesterol, trigliserid, albümin, kan üre nitrojen, kreatinin ve total protein) otoanalizör cihazında ölçüldü. Bulgular: Deney grupları arasında hematolojik ve biyokimyasal parametrelerde önemli bir farklılık belirlenemedi. Sonuç: Koyunlarda 2.5 mg/kg ve 5 mg/kg dozlarda damar içi yolla enrofloksasinin tekrarlayan uygulamalarının hematolojik ve biyokimyasal parametrelerde olumsuz bir etkiye yol açmadığı kanaatine varılmıştır.Öğe Pharmacokinetics of meloxicam following intravenous administration at different doses in sheep(Wiley, 2024) Gungor, Huseyin; Corum, Orhan; Corum, Duygu Durna; Kumru, Alper Serhat; Yilmaz, Gokhan; Coskun, Devran; Coskun, AlparslanThe aim of this study is to determine the pharmacokinetic change after intravenous administration of meloxicam at doses of 0.5, 1 and 2 mg/kg to sheep. The study was carried out on six Akkaraman sheep. Meloxicam was administered intravenously to each sheep at 0.5, 1, and 2 mg/kg doses in a longitudinal pharmacokinetic design with a 15-day washout period. Plasma concentrations of meloxicam were determined using the high performance liquid chromatography-ultraviolet, and pharmacokinetic parameters were evaluated by non-compartmental analysis. Meloxicam was detected up to 48 h in the 0.5 mg/kg dose and up to 96 h in the 1 and 2 mg/kg doses. As the dose increased from 0.5 to 2 mg/kg, terminal elimination half-life, and dose normalized area under the concentration versus time curve increased and total clearance decreased. Compared to the 1 mg/kg dose, it was determined that V(dss )decreased and C-0.083h increased in the 2 mg/kg dose. Meloxicam provided the therapeutic concentration of >0.39 mu g/mL reported in other species for 12, 48 and 96 h at 0.5, 1 and 2 mg/kg doses, respectively. These results show that meloxicam exhibits non-linear pharmacokinetics and will achieve unpredictable plasma concentrations when administered IV for a rapid effect at dose of >= 1 mg/kg in sheep.Öğe Ratlarda Deneysel Oluşturulan Yara Modelinde M.Alba Ekstraktının Etkinliğinin Belirlenmesi(Sivas Cumhuriyet University, 2020) Kara, Haki; Şahin, Mahmut; Güngör, Hüseyin; Kumru, Alper Serhat; Yılmaz, Gökhan; Karayiğit, Mehmet ÖnderAntik çağlardan beri bitkiler çeşitli hastalıkların tedavisinde kullanılmaktadırlar. Morus alba da eskiden beri başta diabet olmak üzere karaciğer, dalak, immun sistem ve kardiyovasküler sistem kaynaklı pek çok hastalığın tedavisinde geleneksel olarak kullanılmaktadır. Bu çalışmada Morus albanın yara iyileşmesi üzerindeki etkileri araştırılmıştır. Çalışmada Wistar cinsi erkek ratlarda oluşturulan yara modelinde kontrol, dekspantenol (%2,5 ve %5) ve Morus alba ekstraktı (%2,5 ve %5) uygulanmıştır. Çalışma süresince 3, 6, 9, 12 ve 15. günlerde kaliper ile ölçümler yapılıp 15. Günde ratlar ötanazi edilerek histopatolojik değerlendirmeler yapılmıştır. Elde edilen veriler ışığında Morus Alba ekstraktlarının kontrol ve dekspantenol verilenlere göre daha hızlı yara iyileşmesi hızı sağladığını göstermiştir. Sonuç olarak Morus alba ekstraktının yara iyileşmesinde kullanılabileceği kanaatine varılmıştır.Öğe The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020) Arslanbas, Emre; Kara, Haki; Turgut, Nergiz Hacer; Gungor, Huseyin; Dogan, Halef Okan; Atasoy, Mustafa Ozan; Kumru, Alper SerhatThis study aims to explore the pharmacodynamics of chelidonine, the active ingredient in greater celandine (Chelidonium majus L.), on in vitro rat bladder and trachea tissue, and evaluate its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activity was measured on primary cell lines obtained from lung and kidney tissue samples. The study has shown that chelidonine has a relaxant effect on bladder and trachea tissues, and it may be mentioned that this effect is produced via muscarinic receptors. In addition, chelidonin caused a statistically insignificant increase in cell viability in primary lung and kidney cell lines at increasing doses (1 and 4 mu g/mL), but this increase remained at the control group level. In contrast, chelidonin caused a significant decrease in cell viability at the same cell lines at doses of 8 and 16 mu g/mL. In conclusion, it is suggested that greater celandine, which is used in folk medicine, and its active ingredient chelidonine might have beneficial effects on asthma, urinary incontinence and other urinary tract and respiratory diseases among others.Öğe The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines(2020) Arslanbaş, Emre; Kara, Haki; Turgut, Nergiz Hacer; Güngör, Hüseyin; Doğan, Halef Okan; Atasoy, Mustafa Ozan; Kumru, Alper SerhatThis study aims to assess the pharmacodynamics of aucubin, the active ingredient in plantain (Plantago), in vitrorat bladder and trachea tissues, and explore its cell protective effects on primary lung and kidney cell lines. The study wascarried out by repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca ++ in a calcium-freeenvironment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamineon trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activities was measured in primarycell lines. The results indicated that aucubin had a relaxant effect on urinary bladder and trachea tissues. It was conceivedthat aucubin acted as a cholinergic antagonist through different subreceptors (muscarinic-3 receptors). The results alsoindicated that aucubin caused a statistically insignificant increase in remaining at the level of the control group in cellviability in the primary lung and kidney cell lines at increasing concentrations (1-10 µM), while causing a significant reducein cell viability at 20 and 50 µM concentrations in the same cell lines. In conclusion, it is suggested that plantain, which isused in folk medicine, and its active ingredient aucubin might have beneficial effects on bronchoconstriction and otherrespiratory conditions, as well as on pulmonary and renal diseases, urinary incontinence, etcÖğe The Effect of Repeated Administration at Different Doses of Enrofloxacin on Hematological and Biochemical Parameters in Sheep(Balıkesir Üniversitesi, 2021) Güngör, Hüseyin; Kumru, Alper Serhat; Yılmaz, Gökhan; Coşkun, AlparslanAim: The aim of this study is to determine the effects of repeated administration (once a day for five days) of intravenous at different doses (2.5-5 mg/kg) of enrofloxacin on hematological and biochemical parameters in healthy sheep. Materials and Methods: Twelve Akkaraman female sheep were used in this study. The animals were divided into two groups of 6 each. One group was injected intravenously (v. jugularis) with at the dose of 2.5 mg/kg enrofloxacin, and the other was injected intravenously with at the dose of 5 mg/kg enrofloxacin for 5 days. Blood samples were collected at 0 (Control) and 120 hours. Hematological parameters (WBC, RBC, HGB, HCT ve PLT) were determined in a blood cell counter and biochemical parameters (AST, ALT, ALP, GGT, cholesterol, triglyceride, albumin, blood urea nitrogen, creatinine, and total protein) were determined in an autoanalyzer. Results: There was no significant difference in hematological and biochemical parameters between the two groups. Conclusion: It has been shown to results of the study that repeated administration of intravenous at doses of 2.5 mg/kg and 5 mg/kg enrofloxacin in sheep does not cause a negative effect on clinical hematological and biochemical parameters. Keywords: Enrofloxacin, Sheep, Hematology, Biochemical Parameters
