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Öğe Anticancer Effect of COX-2 Inhibitor DuP-697 Alone and in Combination with Tyrosine Kinase Inhibitor (E7080) on Colon Cancer Cell Lines(ASIAN PACIFIC ORGANIZATION CANCER PREVENTION, 2014) Altun, Ahmet; Turgut, Nergiz Hacer; Kaya, Tijen TemizColorectal cancer remains one of the most common types of cancer and a leading cause of cancer death worldwide. In this study, we aimed to investigate effects of DuP-697, an irreversible selective inhibitor of COX-2 on colorectal cancer cells alone and in combination with a promising new multi-targeted kinase inhibitor E7080. The HT29 colorectal cancer cell line was used. Real time cell analysis (xCELLigence system) was conducted to determine effects on colorectal cell proliferation, angiogenesis was assessed with a chorioallantoic membrane model and apoptosis was determined with annexin V staining. We found that DuP-697 alone exerted antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. For the antiproliferative effect the half maximum inhibition concentration (IC50) was 4.28x10(-8) mol/L. Antiangiogenic scores were 1.2, 0.8 and 0.5 for 100, 10 and 1 nmol/L DuP-697 concentrations, respectively. We detected apoptosis in 52% of HT29 colorectal cancer cells after administration of 100 nmol/L DuP-697. Also in combination with the thyrosine kinase inhibitor E7080 strong antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells were observed. This study indicates that DuP-697 may be a promising agent in the treatment of colorectal cancer. Additionally the increased effects observed in the combination with thyrosine kinase inhibitor give the possibility to use lower doses of DuP-697 and E7080 which can avoid and/or minimize side effects.Öğe Effect of black mulberry (Morus nigra) extract treatment on cognitive impairment and oxidative stress status of d-galactose-induced aging mice(TAYLOR & FRANCIS LTD, 2016) Turgut, Nergiz Hacer; Mert, Derya Guliz; Kara, Haki; Egilmez, Hatice Reyhan; Arslanbas, Emre; Tepe, Bektas; Gungor, Huseyin; Yilmaz, Nese; Tuncel, Necati BarisContext: Morus nigra L. (Moraceae) has various uses in traditional medicine. However, the effect of M. nigra on cognitive impairment has not been investigated yet. Objective: The objective of this study is to determine the phenolic acid content and DNA damage protection potential of M. nigra leaf extract and to investigate the extract effect on cognitive impairment and oxidative stress in aging mice. Materials and methods: Phenolic acid content was determined by quantitative chromatographic analysis. DNA damage protection potential was evaluated on pBR322 plasmid DNA. Thirty-two Balb-C mice were randomly divided into four groups (control, d-galactose, d-galactose + M. nigra 50, and d-galactose + M. nigra 100). Mice were administered d-galactose (100 mg/kg, subcutaneous) and M. nigra (50 or 100 mg/kg, orally) daily for 8 weeks. Behavioral responses were evaluated with Morris water maze. Activities of antioxidant enzymes and levels of malondialdehyde (MDA) were assayed in serum, brain, and liver. Results: In extract, vanillic (632.093 mu g/g) and chlorogenic acids (555.0 mu g/g) were determined. The extract between 0.02 and 0.05 mg/mL effectively protected all DNA bands against the hazardous effect of UV and H2O2. Morus nigra significantly improved learning dysfunctions (p< 0.01), increased memory retention (p < 0.01), reduced MDA levels (p < 0.05), and elevated SOD, GPx, and CAT activities (p < 0.05) compared with the d-galactose group. Discussion and conclusion: These results show that M. nigra has the potential in improving cognitive deficits in mice and that M. nigra may be useful to suppress aging, partially due to its scavenging activity of free radicals and high antioxidant capacity.Öğe The effect of octreotide, an analog of somatostatin, on bleomycin-induced interstitial pulmonary fibrosis in rats(TAYLOR & FRANCIS LTD, 2013) Tug, Tuncer; Kara, Haki; Karaoglu, Aziz; Karatas, Fikret; Turgut, Nergiz Hacer; Ayan, Erhan; Boran, Cetin; Tug, EsraIn this study, octreotide (OCT), a synthetic sonnatostatin analog, was tested for its beneficial effects in the prevention of interstitial pulmonary fibrosis (IPF) induced by bleomycin (BLM) in rats by histological examination and by evaluating tissue OH-proline levels. Thirty male Wistar rats were divided randomly into three groups: group I: intratracheal (i.t.) BLM (7.5 mg/kg, single dose) + saline solution [0.9% NaCl, subcutaneously (s.c.), once-daily for 7 days]; group II: i.t. BLM (7.5 mg/kg, single dose) + OCT acetate (82.5 mu g/kg, s.c., once-daily for 7 days); and the control group. At the end of the 7 days, lung tissues were excised and examined by histopathological methods. Levels of tissue hydroxyproline (OH-proline) were determined. BLM administration resulted in prominent histopathologic findings, such as diffuse alveolar damage and interstitial pulmonary fibrosis, as well as a significant increase in OH-proline level, as compared to controls. OCT application explicitly attenuated the histopathologic changes to a significant extent. OCT decreased paranchymal fibrosis and structural deformities in BLM-induced lung fibrosis. These results suggest that OCT administration to rats with BLM-induced IPF has a protective effect. Further studies are necessary to reveal the molecular mechanism(s) of OCT-induced protective effect.Öğe The effect of sildenafil on the altered thoracic aorta smooth muscle responses in rat pre-eclampsia model(ELSEVIER SCIENCE BV, 2008) Turgut, Nergiz Hacer; Temiz, Tijen Kaya; Bagcivan, Ihsan; Turgut, Butent; Gulturk, Sefa; Karadas, BarisThe pathophysiology of pre-eclampsia is still unknown thus effective primary prevention is not possible at the stage. The present study was conducted to research the smooth muscle responses in the pre-eclampsia model with suramin treated rats and the effect of phosphodiesterase-5 (PDE5) inhibitor on these responses. Rats of three groups; control, suramin and suramin+sildenafil were given intraperitoneal injections of saline, suramin or sildenafil citrate. Suramin injections caused increased blood pressure, protein in urine and caused fetal growth retardation.The use of sildenafil citrate straightened significantly both blood pressure and average fetus weight, but did not reach to control values. At the end of pregnancy, thoracic aorta rings were exposed to contractile and relaxant agents. KCl contraction responses, sodium nitroprusside and papaverine relaxation responses were similar in three groups. Contraction responses of phenylephrine, increased significantly in suramin group. Relaxation responses of acethylcholine and bradykinin decreased in suramin group. The use of sildenafil citrate partially straightened both relaxation and contraction responses, but did not reach to control values. In all groups in the presence of L-nitromonomethylarginine (L-NAME), 1H-(1, 2, 4) oxadiazole (4, 3-a) guinoxalin-1-one (ODQ) and indomethacin decreased the relaxation responses of acetylcholine and bradykinin. The cyclic guanosine monophosphate (cGMP) content of thoracic aorta tissue was determined by radioimmunoassay technique. The content of cGMP in suramin group decreased and use of sildenafil citrate increased the cGMP content but did not reach to control values. We conclude that in pre-eclampsia, the increase of contraction responses, the decrease of relaxation responses and the decrease of cGMP content can depend on insufficiency about synthesis or release of relaxant factors which was released from the vessel endothelium. The results in this study show that in pre-eclampsia: PDE5 inhibitors enhance endothelial function and may be used for protection. Further studies are needed to clear the efficiency and safety of PDE5 inhibitors. (c) 2008 Elsevier B.V. All rights reserved.Öğe Enoxaparin pretreatment alleviates pentylenetetrazol-induced epileptic seizures in Wistar rats(Univ Zulia, Facultad Ciencias Veterinarias, 2024) Gungor, Huseyin; Turgut, Nergiz HacerEpilepsy, is a prevalent neurological disorder characterized by recurring seizures. A low molecular weight heparin enoxaparin has multifaceted properties. In addition to its anticoagulant activity, enoxaparin has demonstrated anti-inflammatory, antioxidant and anti-apoptotic effects. Accordingly, the purpose of this study was to evaluate the protective effect of enoxaparin against seizures, oxidative stress, proinflammatory cytokines, apoptosis, brain-derived neurotropic factor (BDNF) concentrations and cognitive impairment in pentylenetetrazole (PTZ) induced kindling in Wistar rats. Twenty-four rats divided into 4 groups (Control, PTZ, ENX250+PTZ, ENX500+PTZ) were used. Enoxaparin (250 and 500 IUkg(-1) , intraperitoneal-ip-) or vehicle (saline) were given to rats for 5 days. On the fifth day, 30 min after drug administration, PTZ (45 mgkg(-1) , ip) was given to cause seizures. Behavioral seizure parameters were evaluated by video recording. A behavioral test, passive avoidance test was performed. PTZ administration decreased total antioxidant status (TAS) while increased total oxidant status (TOS) both in hippocampus and cortex. Furthermore, PTZ induced elevated levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), BDNF, caspase-3, and caspase-9. Pretreatment with enoxaparin decreased the levels of these parameters and TOS, while increased TAS. Enoxaparin pretreatment significantly decreased the epileptic seizure scores according to the Racine scale, increased first myoclonic jerk (FMJ) time and the test trial time in passive avoidance test. These results indicate that enoxaparin (250 and 500 IUkg(-1)) at both doses has promising protective effect against PTZ induced epilepsy by improving memory impairment, inflammation, oxidative stress and apoptosis. This positive effect was more prominent at 500 IUkg(-1) dose of enoxaparin.Öğe Evaluation of myocardial viability with thallium-201 infusion MPSPECT after oral glucose application in patients with chronic coronary artery disease(LIPPINCOTT WILLIAMS & WILKINS, 2009) Hasbek, Zekiye; Turgut, Bulent; Erselcan, Janer; Yalta, Kenan; Tandogan, Izzet; Ozer, Gurkan; Ozdemir, Umit; Turgut, Nergiz HacerAim The aim of this study was to evaluate the myocardial viability in nondiabetic patients with chronic coronary artery disease (CCAD) or past myocardial infarction (MI), using thallium-201 infusion myocardial perfusion single-photon emission computed tomography (MPSPECT) imaging after oral glucose application (Glu + Tl-201-infusion). Materials and methods In this study, 33 nondiabetic patients (three female, 30 male, mean age: 55.24 +/- 11 years, range: 33-77 years) with MI history or known CCAD were included. Rest/redistribution/24 h-late-MPSPECT imaging was performed for all patients. In all patients in whom fixed perfusion defect was observed on any wall of the left ventriculi, after 24 h-late-MPSPECT imaging, 75g oral glucose was given. Thirty minutes later, 1 mCi thallium-201 in 100 ml of physiological saline solution was applied in a period of 20 min by slow infusion. After infusion at the 10th minute, MPSPECT imaging was performed. Perfusion was evaluated visually for a total of 3432 segments with the 26-segment 5-point scoring technique. Scoring measured perfusion as 0=no perfusion defect, 1=mildly reduced, 2=moderately reduced, 3=severely reduced, and 4=absent uptake. Scores '0 and 1' were considered normal and scores '2-4' were considered abnormal. Results For serum insulin levels measured after glucose application, a significant increase was determined, according to the period before glucose application (P<0.001). When compared with rest MPSPECT images, segmental perfusion improvement both in redistribution and in the 24 h-late-MPSPECT images were 16.3 and 18.3%, respectively. This ratio was found to be 272% for Glu + Tl-201-infusion images. The ratios of segments in which perfusion was worsening were calculated to be 9.4, 14.5, and 7.3%, respectively, for redistribution, 24 h-late-MPSPECT, and Glu + Tl-201-infusion images. When this evaluation was made for all three vessel areas, again the highest perfusion improvement and the lowest perfusion worsening were detected for Glu + Tl-201-infusion images. In addition, when this evaluation was made for the three vessel areas according to the coronary narrowing degree, again the highest perfusion improvement was detected for Glu + Tl-201-infusion images, in segments in the left anterior descending artery, and right coronary artery areas with >= 90% narrowing. In rest images, in segments with segmental scores of 3 and 4, when the total reversibility ratio was evaluated, this ratio was calculated to be 0.7% for redistribution images and 4.5% for 24 h-late-M PSPECT. The highest total reversibility ratio in these segments was detected with Glu + Tl-201-infusion images to be 10.3%. When we evaluated the patients with respect to the MI history time, the highest segmental perfusion improvement was detected in patients with 0-3 months of MI history. Conclusion We conclude that in nondiabetic patients who are known to have CCAD or past MI history, Glu + (TI)-T-201-infusion is an easily applicable method that gives better results for the evaluation of myocardial viability. Nucl Med Commun 30:779-788 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.Öğe Examination of ototoxicity induced by imatinib, being a tyrosine kinase inhibitor: An experimental study(Wolters Kluwer Medknow Publications, 2020) Altuntas, Emine Elif; Durmus, Kasim; Bora, Adem; Turgut, Nergiz Hacer; Terzi, Hatice; Kutluhan, AhmetObjectives: Two rats were excluded from the study. Because otitis media developing one rat in Group C (7th dayof the experiment) and bleeding-related death one rat in Group I-50 (14th day of the experiment). While the side effects of imatinib are investigated in the literature, it is remarkable that the case reports suggesting an ototoxic side effect also take place among the publications. The aim of this study was to investigate whether or not imatinib has any ototoxic effect on rats via auditory brainstem response (ABR) responses. Materials and Methods: Rats were divided into three groups as Group C (0.25 mL/kg/day), Group I-30 (30 mg/kg/day), and Group I-50 (50 mg/kg/day). In the ABR record, hearing threshold, latency, amplitude, and interpeak latency values on test days were recorded and assessed. Results: In the assessment made in terms of mean V Wave latency within the group, a difference was determined at all stimulus intensities at 8 kHz in Group I-50 (P < 0.05). In the within-group assessment performed in terms of mean Wave III latency, there were differences in Groups I-30 and I-50 (P < 0.05). In the within-group assessment performed in terms of I-III interpeak latency mean values, there was a difference at 4 kHz and 70 dB in Group I-30 (P < 0.05). In the within-group assessment in terms of mean III-V interpeak latency values, the difference between the groups was significant on the 7th day at 6 kHz and 50 dB (P = 0.044) and on the 14th day at 8 kHz and 70 dB (P = 0.036). In the within-group assessment in terms of Wave I amplitude mean values, the change in the amplitude values at 4 kHz (P = 0.003) and 6 kHz (P = 0.018) in Group I-50 was significant. Conclusion: It was observed that imatinib application caused elongation in latency and interpeak latency values and changes in amplitude values. These differences were not enough to state that imatinib is having an ototoxic side effect.Öğe Histopathological evaluation of the effect of locally administered strontium on healing time in mandibular fractures: An experimental study(WROCLAW MEDICAL UNIV, 2017) Durmus, Kasim; Turgut, Nergiz Hacer; Dogan, Mehtap; Tuncer, Ersin; Ozer, Hatice; Altuntas, Emine Elif; Akyol, MelihBackground. Mandibular fractures are the most common facial fractures. They can be treated by conservative techniques or by surgery. The authors hypothesized that the application of a single local dose of strontium chloride would accelerate the healing of subcondylar mandibular fractures, shorten the recovery time and prevent complications. Objectives. The aim of the present pilot study was to evaluate the effects of a single local dose of strontium chloride on the healing of subcondylar mandibular fractures in rats. Material and methods. This randomized experimental study was carried out on 24 male Wistar albino rats. The rats were randomly divided into 3 groups: experimental group 1, receiving 3% strontium chloride; experimental group 2, receiving 5% strontium chloride; and the control group. A full thickness surgical osteotomy was created in the subcondylar area. A single dose of strontium solution (0.3 cc/site) was administered locally by injection on the bone surfaces of the fracture line created. Nothing was administered to the control group. The mandibles were dissected on postoperative day 21. The fractured hemimandibles were submitted to histopathological examination. Results. The median bone fracture healing score was 9 (range: 7-9) in experimental group 1; 8 (range: 7-10) in experimental group 2; and 7.50 (range: 7-8) in the control group. When the groups were compared in terms of bone healing scores, there was a statistically significant difference between experimental group 1 and the control group (p < 0.05). Conclusions. This study is the first to show that local strontium may have positive effects on the healing of subcondylar mandibular fractures. In the authors' opinion, 3% strontium was beneficial for accelerating facial skeleton consolidation and bone regeneration in rat subcondylar mandibular fractures. This treatment procedure may be combined with closed fracture treatment or a conservative approach.Öğe The influence of quercetin on recognition memory and brain oxidative damage in a ketamine model of schizophrenia(TAYLOR & FRANCIS LTD, 2019) Mert, Derya Guliz; Turgut, Nergiz Hacer; Arslanbas, Emre; Gungor, Huseyin; Kara, HakiOBJECTIVE: The aims of this study were to investigate the protective effect of quercetin on changes in recognition memory as assessed by the novel object recognition (NOR) test, as well as on changes in the oxidative stress levels in the hippocampus and prefrontal cortex, produced in a model of memory impairment in schizophrenia induced by administration of a subanesthetic dose of ketamine. METHODS: A total of 40 Balb-C mice were randomly divided into five groups (Corn oil + Saline, Quercetin 50 + Saline, Corn oil + Ketamine, Quercetin 25 + Ketamine, Quercetin 50 + Ketamine). Corn oil and Quercetin (25 or 50 mg/kg/day) was given by orogastric gavage once daily for 21 days. Corn oil was chosen as the vehicle and administered at the same volume as quercetin. Ketamine was injected at a dose of 25 mg/kg intraperitoneally (i.p.) for a period of 7 days starting from the 15th day. Behavioural responses were evaluated with the NOR test. The activity levels of antioxidant enzymes and levels of malondialdehyde (MDA) were assayed in the prefrontal cortex and hippocampus. RESULTS: The time of exploration of the novel object was longer than T-F (time to explore the familiar object) in the Corn oil + Saline and Quercetin 50 + Saline groups in NOR Test-1 (p < .05). The discrimination ratios of the Quercetin 50 + Ketamine and Corn oil + Ketamine groups were significantly lower than that of the Quercetin 50 + Saline group (p < .05). The discrimination ratios of the Quercetin 50 + Ketamine and Corn oil + Saline groups were significantly lower than that of the Quercetin 50 + Saline group (p < .05). The time of exploration of the novel object was longer than T-F in the Corn oil + Saline and Quercetin 50 + Ketamine groups in NOR Test-2 (p < .05). The discrimination ratios of the Corn oil + Ketamine and Quercetin 25 + Ketamine groups were significantly lower than those of the Quercetin 50 + Ketamine group (p < .05). Quercetin at 50 mg/kg reduced the MDA levels and elevated the SOD and GPx activity compared to the Corn oil + Ketamine group. CONCLUSION: These results show that quercetin has the potential to improve cognitive deficits in mice and that quercetin may be useful for treating the symptoms of schizophrenia, partially due to its ability to scavenge free radicals and its high antioxidant capacity.Öğe Investigation of the role of the NO-cGMP pathway on YC-1 and DEA/NO effects on thoracic aorta smooth muscle responses in a rat preeclampsia model(CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 2013) Turgut, Nergiz Hacer; Temiz, Tijen Kaya; Turgut, Bulent; Karadas, Baris; Parlak, Mesut; Bagcivan, IhsanThe present study was designed to investigate the effects of YC-1, a nitric oxide (NO)-independent soluble guanylate cyclase (sGC) activator, and DEA/NO, a NO donor, on smooth muscle responses in the preeclampsia model with suramin-treated rats and on the levels of cyclic guanosine monophosphate (cGMP) of thoracic aorta rings isolated from term-pregnant rats. Rats of 2 groups, control group and suramin group, were given intraperitoneal injection of saline or suramin, respectively. Suramin injection caused increased blood pressure, protein in urine, and fetal growth retardation. Thoracic aorta rings were exposed to contractile and relaxant agents. KCl contraction and papaverine relaxation responses were similar. Relaxation responses of YC-1 and DEA/NO decreased in suramin group. In both groups in the presence of ODQ, a sGC inhibitor, the relaxation responses of YC-1 and DEA/NO decreased. The cGMP content was determined by radioimmunoassay technique. The content of cGMP in the suramin group decreased. In the presence of YC-1 and DEA/NO in both groups, cGMP content increased, but in ODQ-added groups, there was a significant decrease. We conclude that in preeclampsia, the decrease of relaxation responses and the decrease of cGMP content could be due to the reduction in stimulation of sGC and the decrease in cGMP levels.Öğe Perspectives on reasons of medication nonadherence in psychiatric patients(DOVE MEDICAL PRESS LTD, 2015) Mert, Derya Guliz; Turgut, Nergiz Hacer; Kelleci, Meral; Semiz, MuratPurpose: This study was carried out to evaluate factors resulting in medication nonadherence within 6 months before admission to the psychiatric service of our hospital for bipolar disorder, schizophrenia/schizoaffective disorder, depression, and other psychiatric diseases. Patients and methods: Two hundred and three patients admitted to the Psychiatry Service of the Medical Faculty were included in this study. Sociodemographic parameters and clinical findings within 6 months before admission and patients' views on reasons of medication nonadherence were examined. Results: Patients were classified into four groups according to their diagnosis: bipolar disorder (n=68, 33.5%), schizophrenia/schizoaffective disorder (n=59, 29.1%), depression (n=39, 19.2%), and others (n=37, 18.2%). The ratio of medication nonadherence was higher in the bipolar disorder group when compared to the groups with schizophrenia/schizoaffective disorder, depression, and other disorders (12.1%, 18.2%, and 24.2% vs 45.5%); however, the ratio of medication nonadherence was similar in schizophrenia/schizoaffective disorder, depression, and the others group. In logistic regression analysis, irregular follow-up (odds ratio [OR]: 5.7; 95% confidence interval [CI]: 2.92-11.31) and diagnosis (OR: 1.5; 95% CI: 1.07-1.95) were determined to be important risk factors for medication nonadherence. The leading factors for medication nonadherence were: "not willing to use medication", "not accepting the disease", and "being disturbed by side effects" in the bipolar disorder group, "not accepting the disease" in the schizophrenia/schizoaffective disorder group, "feeling well" in the depression group, and "being disturbed by side effects" in the other diseases group. Conclusion: Medication nonadherence is an important problem in psychiatric patients and should be dealt with by taking into account the diagnosis, attendance to follow-up appointments, and the patient's attitude. Ensuring regular attendance to follow-up appointments, adjusting the management plan according to the diagnosis, and improving their thoughts about resistance to medication can be beneficial in terms of medication adherence.Öğe The seroprevalence of Anaplasma phagocytophilum in humans from two different climatic regions of Turkey and its co-seroprevalence rate with Borrelia burgdorferi(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2011) Gunes, Turabi; Poyraz, Omer; Atas, Mehmet; Turgut, Nergiz HacerAim: To investigate the seroprevalence of Anaplasma phagocytophilum in people living in 2 different climatic regions and to evaluate the co-seroprevalence rate of A. phagocytophilum with Borrelia burgdorferi. Sinop and Tokat provinces, both in the middle Black Sea region of Turkey, have distinct climatic features. Materials and methods: In 2006-2007, serum samples were collected from people living in rural areas of Tokat and Sivas, and anti-A. phagocytophilum IgG antibodies were explored by the IFA method. Positive samples were further investigated for the possible presence of B. burgdorferi IgG antibodies. Results: A. phagocytophilum seropositivity was found in 29 (10.62%) out of 273 serum samples in Sinop and 21 (5.77%) out of 364 serum samples in Tokat (P = 0.035). Co-seroprevalence for A. phagocytophilum and B. burgdorferi was found to be 3.30% in Sinop and 0.55% in Tokat (P = 0.012). Conclusion: The current study suggests that A. phagocytophilum infections can be seen in humans from different climatic regions of Turkey. Both the seroprevalence of A. phagocytophilum and the possibility of mixed infections of A. phagocytophilum and B. burgdorferi are higher in places with more suitable habitats for Ixodes ricinus ticks.Öğe The seroprevalence of Rickettsia conorii in humans living in villages of Tokat Province in Turkey, where Crimean-Congo hemorrhagic fever virus is endemic, and epidemiological similarities of both infectious agents(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2012) Gunes, Turabi; Poyraz, Omer; Atas, Mehmet; Turgut, Nergiz HacerAim: Tokat Province is an epicenter for Crimean-Congo hemorrhagic fever virus (CCHFV) in Turkey. The aim of this study was to investigate the seroprevalence of Rickettsia conorii and to clarify the epidemiological similarities between CCHFV and R. conorii in Tokat Province. Materials and methods: The prevalence of antibodies reactive with R. conorii was examined by ELISA in 364 sera, 151 of which were seropositive for CCHFV. Results: The overall prevalence of antibodies reactive with R. conorii was 36.81%. The prevalence of antibodies to R. conorii infection was higher in humans who showed CCHFV seropositivity than seronegativity, 52.32% and 25.82%, respectively (P = 0.001). A significant difference in seroprevalence was found between groups who had a history of tick bite and who did not, 41.52% and 29.29%, respectively (P = 0.019). Conclusion: Our data show that people who are a risk group for CCHFV are likely to be a risk group for R. conorii.Öğe Strontium chloride hexahydrate as a candidate molecule for long-term treatment of allergic rhinitis(MEDKNOW PUBLICATIONS & MEDIA PVT LTD, 2017) Altuntas, Emine Elif; Turgut, Nergiz Hacer; Durmus, Kasim; Dogan, Omer Tamer; Akyol, MelihBackground & objectives: Neurogenic inflammation plays a role in the pathophysiology of allergic rhinitis (AR). Strontium salts are highly effective in reducing the sensory irritation. This study was aimed to investigate the efficacy of strontium chloride (SC) on AR symptoms based on the duration of SC use before the symptoms begin. Methods: Wistar albino rats (n= 18) were randomly divided into three groups: Group 1, received 1 mu g mometasone furoate (MF); Group 2, three per cent SC; and Group 3 received five per cent SC (2 mu l/site). Drugs were administered to the each nasal cavity for three weeks every morning. On the days 7, 14 and 21, histamine dihydrochloride (HD) 5 mu mol (2 mu l/site) was administered and the frequencies of nasal rubbing and sneezing were counted for 15 min. Results: After 7, 14 and 21 day medication period, the groups were compared in terms of the frequency of sneezing and nasal rubbing following HD. There was a significant difference among the groups in terms of the frequency of sneezing on the day 7 (P< 0.05). Intragroup comparisons for the nasal rubbing showed significance (P< 0.05). In Group 3, there was a decrease in the number of nasal rubbings on the day 14 and 21; however, the difference was not significant. Interpretation & conclusions: Our results showed that three and five per cent SC were less effective than MF for sneezing during the first week, but the efficiency was equal to that of MF after the first 14 days. Long-term use of SC was as effective as MF on nasal rubbing. SC can be as effective as MF on both sneezing and nasal rubbing on regular use over three weeks.Öğe Suraminle oluşturulan sıçan preeklampsi modelinde damar düz kas cevaplarında oluşan değişiklikler üzerine fosfodiesteraz-5 inhibisyonunun etkisi(Cumhuriyet Üniversitesi, 2007) Turgut, Nergiz Hacer; Temiz, Tijen KayaPreeklampsi, hem maternal hem de fetal ölüme yol açabilen ve hamilelerin yaklaşık %5'inde görülebilen önemli bir hastalıktır. Preeklampsinin fizyopatolojisi henüz tam olarak anlaşılamamıştır ve bu nedenle tedavilerin hiçbiri hastalığı önlemede tam olarak etkili olamamaktadır. Bu çalışmada suraminle oluşturulan sıçan preeklampsi modelinde damar düz kas yanıtlarında oluşan değişiklikler ve bu değişiklikler üzerine fosfodiesteraz-5 (PDE-5) inhibisyonunun etkisini araştırmayı amaçladık. Çalışmamızda literatürde preeklampsinin pek çok klinik özelliğini oluşturmada başarılı olduğu belirtilen suramin ile sıçanlarda preeklampsi modeli oluşturulmuştur. Hamile sıçanlar kontrol (n=10), preeklampsi (suramin) (n=10) ve suramin+sildenafil sitrat grubu (n=10) olmak üzere üç gruba ayrılmıştır. Kontrol grubundaki sıçanlara gebeliklerinin 10 ve 11. gününde intraperitoneal (i.p.) 0.3 ml serum fizyolojik, preeklampsi ve suramin+sildenafil sitrat grubundaki sıçanlara gebeliklerinin 10 ve 11.gününde 100 mg/kg i.p. tek doz suramin, suramin+sildenafil sitrat grubundaki sıçanlara suramin uygulamasına ilaveten gebelik sonuna kadar 5 mg/kg sildenafil sitrat i.p. uygulanmıştır. Preeklampsi annede artan kan basıncı ve proteinüri, bebekte ise genellikle bunlara eşlik eden büyüme geriliğiyle karakterize bir hastalık olması nedeniyle çalışmada her 3 gruptaki sıçanların kan basınçları ölçülmüş, idrar örneklerinde protein miktarına bakılmış ve fetus ağırlıkları tartılarak kaydedilmiştir. Kuyruk kaf yöntemiyle ölçülen kan basıncı düzeyleri kontrol grubuyla karşılaştırıldığında preeklampsi grubunda daha fazla olmak üzere deney gruplarında anlamlı bir şekilde artmıştır (p<0.05). İdrar örneklerinde protein miktarına ?dipstick? yöntemiyle bakılmıştır. Preeklampsi grubu ve suramin+sildenafil sitrat grubunda idrar örneklerinde protein saptanmıştır. Ortalama fetus ağırlığı kontrol grubuna göre preeklampsi ve suramin+sildenafil sitrat grubunda anlamlı bir şekilde azalmıştır (p<0.05). Ortalama fetus ağırlığı preeklampsi ve suramin+sildenafil sitrat grupları arasında karşılaştırıldığında ise preeklampsi grubuna göre suramin+sildenafil sitrat grubunda anlamlı bir artma saptanmıştır (p<0.05). Gebeliğin sonunda (21.gün) her üç gruptaki sıçanlardan elde edilen torasik aorta şeritleri, organ banyosuna kasıcı ve gevşetici maddelerle maruz bırakılmak için asılmış ve elde edilen yanıtlar karşılaştırılmıştır. KCl kasılma yanıtları ile endotelden bağımsız gevşeme yapan sodyum nitroprussiyat gevşeme yanıtları her 3 grupta da benzer olarak bulunmuştur (p>0.05). Fenilefrin, arjinin-vazopressin ve endotelin-1 kasılma yanıtları preeklampsili grupta, kontrol grubu ile karşılaştırıldığında, anlamlı bir şekilde artmıştır (p<0.05). Asetilkolin ve bradikinin gevşeme yanıtları ise preeklampsili grupta, kontrol grubu ile karşılaştırıldığında, anlamlı bir şekilde azalmıştır (p<0.05). Sildenafil uygulaması hem gevşeme hem de kasılma yanıtlarını preeklampsi grubu ile karşılaştırıldığında anlamlı şekilde düzeltmiş (p<0.05), ancak kontrol değerlere ulaştırmamıştır. Her 3 grupta da asetilkolin ve bradikinin gevşeme yanıtları L-Nitro monometil arjinin (L-NAME), 1H-[1,2,4] Oxadiazole [4,3-a] quinoxalin-1- one (ODQ) ve indometazin varlığında anlamlı bir şekilde azalmıştır (p<0.05). Her 3 gruptan alınan torasik aorta doku örneklerinde siklik guanozinmonofosfat (cGMP) düzeyleri radyoimmünoassay (RIA) yöntemiyle ölçülmüştür. Preeklampsi grubu cGMP düzeyleri kontrol grubuyla karşılaştırıldığında anlamlı bir şekilde azalmıştır (p<0.05). Sildenafil uygulaması ise cGMP düzeylerini kısmen artırmış ancak kontrol değerlere ulaştıramamıştır. Torasik aorta dokusunun patolojik incelemesinde herhangi bir değişikliğe rastlanmamıştır. Bu sonuçlara göre preeklampside torasik aorta düz kasında kasılma yanıtlarındaki artış reseptör sayısında veya duyarlılığında oluşan bir artmaya bağlı olabilir ya da preeklampside endotelden salınan gevşetici faktörlerin bazal salınımındaki bir azalmaya bağlı olabilir. KCl kasılma ve sodyum nitroprussiyat gevşeme yanıtlarının değişmemesi ise torasik aorta düz kas kasılma-gevşeme fonksiyonunun normal olduğunu ve nitrik oksit (NO) / cGMP yolağının sağlam olduğunu düşündürür. Preeklampside gevşeme yanıtlarının azalması endotelde bulunan reseptörlerdeki azalmaya veya reseptör aracılı yanıt olarak oluşan endotelial NO sentez veya salınımındaki yetersizliğe bağlı olabilir. Ayrıca, çalışmamızda preeklampside cGMP düzeylerinin azalması gevşetici faktörlerin bazal ya da indüklenmiş sentez veya salınımındaki azalma görüşünü desteklemektedir. Gevşeme yanıtlarının L-NAME, ODQ ve indometazin varlığında azalması gevşeme yanıtlarının NO ve prostasikline bağlı olduğunu göstermekte ve preeklampside bu sistemlerde sentez veya salınım sırasında bir bozukluk olabileceğini desteklemektedir. Bu çalışmadan elde edilen sonuçlar preeklampside PDE-5 inhibitörlerinin tedavi edici etkiden çok koruyucu etki yapabileceğini düşündürmektedir. PDE-5 inhibitörlerinin yeterlilik ve güvenilirliğini netleştirmek için daha detaylı çalışmalara gereksinim vardır. Anahtar Kelimeler: preeklampsi, endoteliyal disfonksiyon, fosfodiesteraz-5 inhibisyonu, sıçan.Öğe The Effect of Greater Celandine Active Ingredient Chelidonine on Isolated Rat Bladder and Trachea Smooth Muscles and Primary Lung and Kidney Cell Lines(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020) Arslanbas, Emre; Kara, Haki; Turgut, Nergiz Hacer; Gungor, Huseyin; Dogan, Halef Okan; Atasoy, Mustafa Ozan; Kumru, Alper SerhatThis study aims to explore the pharmacodynamics of chelidonine, the active ingredient in greater celandine (Chelidonium majus L.), on in vitro rat bladder and trachea tissue, and evaluate its cell protective effects on primary lung and kidney cell lines. The study was carried out via repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca++ in a calcium-free environment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamine on trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activity was measured on primary cell lines obtained from lung and kidney tissue samples. The study has shown that chelidonine has a relaxant effect on bladder and trachea tissues, and it may be mentioned that this effect is produced via muscarinic receptors. In addition, chelidonin caused a statistically insignificant increase in cell viability in primary lung and kidney cell lines at increasing doses (1 and 4 mu g/mL), but this increase remained at the control group level. In contrast, chelidonin caused a significant decrease in cell viability at the same cell lines at doses of 8 and 16 mu g/mL. In conclusion, it is suggested that greater celandine, which is used in folk medicine, and its active ingredient chelidonine might have beneficial effects on asthma, urinary incontinence and other urinary tract and respiratory diseases among others.Öğe The Effect of Plantain Active Ingredient Aucubin on Isolated Rat Smooth Muscle Tissue and Primary Cell Lines(2020) Arslanbaş, Emre; Kara, Haki; Turgut, Nergiz Hacer; Güngör, Hüseyin; Doğan, Halef Okan; Atasoy, Mustafa Ozan; Kumru, Alper SerhatThis study aims to assess the pharmacodynamics of aucubin, the active ingredient in plantain (Plantago), in vitrorat bladder and trachea tissues, and explore its cell protective effects on primary lung and kidney cell lines. The study wascarried out by repeated applications of acetylcholine, atropine, verapamil and oxybutynin, alongside Ca ++ in a calcium-freeenvironment, on urinary bladder tissue, and repeated applications of acetylcholine, atropine, carbachol and mecamylamineon trachea tissue. At the same time, cell viability and catalase and superoxide dismutase activities was measured in primarycell lines. The results indicated that aucubin had a relaxant effect on urinary bladder and trachea tissues. It was conceivedthat aucubin acted as a cholinergic antagonist through different subreceptors (muscarinic-3 receptors). The results alsoindicated that aucubin caused a statistically insignificant increase in remaining at the level of the control group in cellviability in the primary lung and kidney cell lines at increasing concentrations (1-10 µM), while causing a significant reducein cell viability at 20 and 50 µM concentrations in the same cell lines. In conclusion, it is suggested that plantain, which isused in folk medicine, and its active ingredient aucubin might have beneficial effects on bronchoconstriction and otherrespiratory conditions, as well as on pulmonary and renal diseases, urinary incontinence, etcÖğe Zingerone ameliorates oxidative stress and inflammation in bleomycin-induced pulmonary fibrosis: modulation of the expression of TGF-?1 and iNOS(Springer, 2020) Gungor, Huseyin; Ekici, Mehmet; Karayigit, Mehmet Onder; Turgut, Nergiz Hacer; Kara, Haki; Arslanbas, EmreIdiopathic pulmonary fibrosis (IPF) is an interstitial lung disease with limited treatment options. Zingerone found in ginger (Zingiber officinale L.) has many pharmacological effects, especially antiinflammatory and antioxidant activity. However, the effect of zingerone on pulmonary fibrosis (PF) is not fully known. The aim of this study was to investigate the effect of zingerone on bleomycin (BLM)-induced PF and its underlying mechanisms. Wistar-albino rats were given single dose of BLM (5 mg/kg, intratracheal) or vehicle (saline). In treatment groups, zingerone (50 and 100 mg/kg, p.o.) was administered orally for 14 days after BLM administration. Rats and lung tissue were weighed to determine lung index. Antioxidant, antiinflammatory effects, and hydroxyproline content of zingerone were determined by ELISA method. Pulmonary inflammation, collagen deposition, and fibrosis score were determined with Hematoxylin-Eosin (HxE) and Masson's trichrome staining. Transforming growth factor-beta 1 (TGF-beta 1) and inducible nitric oxide synthase (iNOS) expressions were detected immunohistochemically. BLM administration increased lipid peroxidation (MDA) and decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity. In addition, BLM caused increased levels of tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in bronchoalveolar lavage fluid (BALF) and accumulation of collagen bundles. Zingerone administration decreased collagen accumulation, TNF-alpha and IL-1 beta levels, MDA level, TGF-beta 1, and iNOS expression and increased SOD and GPx activity. Histopathological findings supported the results. These results show that zingerone (50 and 100 mg/kg) at both doses significantly contributes to healing of PF by improving inflammation, oxidative stress, and histopathological alterations and by affecting TGF-beta 1 and iNOS signaling pathways.
