Application of cloud point extraction for residues of chloramphenicol and amoxicillin in milk samples by HPLC–DAD

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dc.contributor.authorSürücü, Bilge
dc.contributor.authorUlusoy, Halil İbrahim
dc.contributor.authorUlusoy, Songül
dc.contributor.authorDemir, Özge
dc.contributor.authorGülle, Sümeyra
dc.date.accessioned2023-04-12T11:26:09Z
dc.date.available2023-04-12T11:26:09Z
dc.date.issued2022tr
dc.departmentEğitim Bilimleri Enstitüsütr
dc.description.abstractA pre-concentration and sensitive determination method for residues of Chloramphenicol (CLP) and Amoxicillin (AMX)in milk samples were developed based on cloud point extraction (CPE) and HPLC–DAD analysis. CLP and AMX moleculeswere extracted to surfactant phases of polyethylene glycol-6000 (PEG-6000) in the presence of pH 7.0 buffer andhigh electrolyte concentration. Experimental variables were examined and optimized such as concentration of electrolyteand surfactant, pH, incubation time, and diluting solvent. In the developed method, determination of CLP and AMX usingHPLC system was carried out by isocratic elution of water:acetonitrile (30:70) mixture. CLP and AMX antibiotic molecules were analyzed by considering peak area obtained by DAD detector at 273 nm and 276 nm, respectively. After optimizationexperimental variables, analytical signals were linear in the range of 10–900 ng mL− 1 and 25–1000 ng mL−1, respectively.The limit of detection values was calculated as 2.98 and 7.46 ng mL−1 while relative standard deviations (RSD %) were lowerthan 4.20% for 100 ng mL−1. Finally, the developed method was successively applied to cow milk samples.tr
dc.identifier.endpage445tr
dc.identifier.scopus2-s2.0-85118415105en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage437tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/13656
dc.identifier.volume248tr
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringertr
dc.relation.ispartofEuropean Food Research and Technologyen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/openAccesstr
dc.titleApplication of cloud point extraction for residues of chloramphenicol and amoxicillin in milk samples by HPLC–DADen_US
dc.typeArticleen_US

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