Design and in vitro evaluation of curcumin-loaded PLGA nanoparticle-embedded sodium alginate/gelatin 3D printed scaffolds for Alzheimer's disease

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dc.authoridABOBAKR, FATIMA/0009-0009-4354-2751
dc.authoriduner, burcu/0000-0003-4691-0432
dc.contributor.authorYekeler, Humeyra Betul
dc.contributor.authorGuler, Ece
dc.contributor.authorBeato, Patricia Santos
dc.contributor.authorPriya, Sushma
dc.contributor.authorAbobakr, Fatima Khaled Mohammed
dc.contributor.authorDogan, Murat
dc.contributor.authorUner, Burcu
dc.date.accessioned2024-10-26T18:11:31Z
dc.date.available2024-10-26T18:11:31Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBackground: Targeted nanoparticles (NPs) are aimed at improving clinical outcomes by enhancing the diagnostic and therapeutic efficacy of drugs in the treatment of Alzheimer's disease (AD). Methods: Curcumin (CUR)-loaded poly-lactic-co-glycolic acid (PLGA) NPs (CNPs) were produced to demonstrate a prolonged release and successfully embedded into 3D printed sodium alginate (SA)/gelatin (GEL) scaffolds that can dissolve rapidly sublingually. Characterization and in vitro activity of the NPs and scaffolds were evaluated. Results: Based on the in vitro drug release studies, 99.6 % of the encapsulated CUR was released in a controlled manner within 18 days for the CNPs. In vitro cell culture studies showed that all samples exhibited cell viability above 84.2 % and no significant cytotoxic effect on SH-SY5Y cells. The samples were analyzed through 2 different pathways by PCR analysis. Real-time PCR results indicated that CNP and CNP-embedded SA/GEL scaffolds (CNPSGS) may show neuroprotective effects by modulating the Wnt/beta-catenin pathway. The gene expression level of beta-catenin slightly increased compared to the gene expression levels of other proteins and enzymes with these treatments. However, the PI3K/Akt/GSK-3 beta signaling pathway was regulated at the same time because of the crosstalk between these 2 pathways. Conclusion: CNPSGS might be an effective therapeutic alternative for AD treatment.
dc.description.sponsorshipTUBITAK 2211-A [TUBITAK 2211-A, TUBITAK 2250]; Council of Higher Education; TUBITAK 2250
dc.description.sponsorshipH.B.Y. acknowledges TUBITAK 2211-A for her scholarship. E.G. ac- knowledges the Council of Higher Education, TUBITAK 2211-A, and TUBITAK 2250 for her scholarships. The authors would like to thank Joel Turner, a Ph.D. student at the UCL Division of Surgery and Inter- ventional Sciences, for the imaging of cell viability by fluorescence microscopy. The authors dedicate this article to the memory of Turkish citizens, who lost their lives in the earthquakes held in Pazarc & imath;k and Elbistan, Kahramanmaras , , Turkiye on February 06, 2023.
dc.identifier.doi10.1016/j.ijbiomac.2024.131841
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid38679260
dc.identifier.scopus2-s2.0-85191722823
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.131841
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30703
dc.identifier.volume268
dc.identifier.wosWOS:001237893900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlzheimer's disease
dc.subjectCurcumin
dc.subject3D printing
dc.titleDesign and in vitro evaluation of curcumin-loaded PLGA nanoparticle-embedded sodium alginate/gelatin 3D printed scaffolds for Alzheimer's disease
dc.typeArticle

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