Investigation of the Presence of Francisella tularensis and Acanthamoeba spp. in the Drinking Water of Sivas Province, Turkey

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dc.authoridAtas, Mehmet/0000-0002-9425-0080
dc.contributor.authorAtas, Mehmet
dc.contributor.authorPoyraz, Omer
dc.date.accessioned2024-10-26T18:00:16Z
dc.date.available2024-10-26T18:00:16Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractIntroduction: Tularaemia is a zoonotic disease caused by Francisella tularensis. F. tularensis subsp. holarctica is primarily isolated in Northern Europe, the Balkans, Turkey, Siberia, and the Far East, and it is mostly responsible for waterborne outbreaks. F. tularensis, which is a facultative intracellular bacterium, may survive inside Acanthamoeba castellani, which is a free-living amoeba in water resources; this symbiotic life allows the bacteria to survive in water and mud for months. In this study, drinking water samples taken during the tularaemia outbreak and non-outbreak regions were compared in terms of the presence of Francisella tularensis and Acanthamoeba spp., and the role of Acanthamoeba was investigated in tularemia outbreaks observed in Turkey. Materials and Methods: This study was conducted in the province of Sivas located in the central Anatolia region in Turkey, and a total of 300 water samples were used. Glucose-cysteine-blood agar (GCBA) and non-nutrient agar (NNA) were used for the isolation of F. tularensis and Acanthamoeba, respectively. Each isolate was identified by PCR technique. Results: A total of 30 (10%) F. tularensis and 28 (9.33%) Acanthamoeba spp. isolates were produced by culture method. All bacterial strains were confirmed with PCR as to be F. tularensis subsp. holarctica. In our study, concurrent presence of F. tularensis and Acanthamoeba were detected in two water samples. There were no statistically significant differences between the water samples taken from outbreak and non-outbreak regions in terms of the presence of Acanthamoeba (p> 0.05). Conclusion: Even though experimental studies have shown that F. tularensis may survive inside Acanthamoeba spp. and its viability continues, our study could not identify a relationship between tularemia outbreak and Acanthamoeba spp. New research on this issue may have a contribution on tularemia epidemiology.
dc.description.sponsorshipScientific Research Project Fund of Cumhuriyet University [T-428]
dc.description.sponsorshipThis work was supported by the Scientific Research Project Fund of Cumhuriyet University under the project number T-428. We would like to thank Dr. Can Bulent KARAKUS, for giving us technical support to present the samples used in our study on the map. The authors would like to thank the Sivas Cumhuriyet University Faculty of Medicine Research Center (CUTFAM) for its technical support.
dc.identifier.doi10.5578/flora.20219917
dc.identifier.endpage171
dc.identifier.issn1300-932X
dc.identifier.issue1
dc.identifier.startpage163
dc.identifier.trdizinid413714
dc.identifier.urihttps://doi.org/10.5578/flora.20219917
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/413714
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27602
dc.identifier.volume26
dc.identifier.wosWOS:000639966700010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherBilimsel Tip Yayinevi
dc.relation.ispartofFlora Infeksiyon Hastaliklari ve Klinik Mikrobiyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFrancisella tularensis
dc.subjectAcanthamoeba
dc.subjectTularemia
dc.subjectTap water
dc.subjectPCR
dc.titleInvestigation of the Presence of Francisella tularensis and Acanthamoeba spp. in the Drinking Water of Sivas Province, Turkey
dc.typeArticle

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