A simplified and optimized protocol for total DNA extraction from insect species: applicable for studying genetic diversity and PCR-based specimen identification via partial amplification of cytochrome oxidase I (COI) gene

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dc.authoridMirmoayedi, alinaghi -- 0000-0002-8026-0296en_US
dc.contributor.authorPaydar, Samira
dc.contributor.authorUcar, Esra
dc.contributor.authorYari, Peyman
dc.contributor.authorSafavi, Seyed Mehdi
dc.contributor.authorKahrizi, Danial
dc.contributor.authorNateqi, Masoud
dc.contributor.authorMirmoayedi, Alinaghi
dc.contributor.authorYari, Kheirollah
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T09:39:05Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T09:39:05Z
dc.date.issued2018
dc.department[Paydar, Samira] Razi Univ, Dept Agron & Plant Breeding, Kermanshah, Iran -- [Ucar, Esra] Cumhuriyet Univ, Sivas Vocat Sch, Dept Crop & Anim Prod, Sivas, Turkey -- [Yari, Peyman -- Yari, Kheirollah] Kermanshah Univ Med Sci, Med Biol Res Ctr, Kermanshah, Iran -- [Yari, Peyman] Inst Higher Educ Jahad e Daneshgahi, Dept Med Plants, Kermanshah Unit, Kermanshah, Iran -- [Safavi, Seyed Mehdi] Islamic Azad Univ, Kermanshah Branch, Dept Agron & Plant Breeding, Kermanshah, Iran -- [Kahrizi, Danial] Kermanshah Univ Med Sci, Fertil & Infertil Res Ctr, Kermanshah, Iran -- [Nateqi, Masoud] Univ Tehran, Fac Agr Sci & Engn, Dept Plant Protect, Karaj, Iran -- [Mirmoayedi, Alinaghi] Razi Univ, Dept Plant Protect, Kermanshah, Iran -- [Yari, Kheirollah] Razi Univ Incubator, Zagros Bioidea Co, Kermanshah, Iranen_US
dc.description.abstractThe efficient DNA extraction from insects has been suggested as a critical and main step affecting molecular entomology for taxonomic identification, the establishment of DNA barcoding library and analysis of genetic diversity relationship between insect populations. For successfully apply these molecular techniques, high-quantity and high-quality of the extracted DNA are required. Several protocols for efficient genomic DNA extraction from insects have been developed. In this research, we represent a rapid, reliable and cost-effective method that it is not reliant on poisonous and enzymatic reagents for DNA extraction from insect tissues. Results showed that high quantity and high-quality of the isolated DNA by this method is suitable and can be used directly for PCR, also is enough to do hundreds of molecular reactions. In conclusion, we described a fast, cost-effective, non-toxic and enzyme-free protocol for high yield genomic DNA extraction from green Lacewings (Chrysoperla carnea) tissues in basic equipment laboratories.en_US
dc.identifier.doi10.14715/cmb/2018.64.12.5en_US
dc.identifier.endpage25en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue12en_US
dc.identifier.pmid30301497en_US
dc.identifier.scopus2-s2.0-85054776309en_US
dc.identifier.scopusqualityQ4
dc.identifier.startpage22en_US
dc.identifier.urihttps://dx.doi.org/10.14715/cmb/2018.64.12.5
dc.identifier.urihttps://hdl.handle.net/20.500.12418/6468
dc.identifier.volume64en_US
dc.identifier.wosWOS:000449068700005en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherC M B ASSOCen_US
dc.relation.ispartofCELLULAR AND MOLECULAR BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA extraction methoden_US
dc.subjectOptimized protocolen_US
dc.subjectChrysopidaeen_US
dc.subjectCytochrome oxidase Ien_US
dc.titleA simplified and optimized protocol for total DNA extraction from insect species: applicable for studying genetic diversity and PCR-based specimen identification via partial amplification of cytochrome oxidase I (COI) geneen_US
dc.typeArticleen_US

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