RNA N6-Methyladenosine Pathway Writer Genes Expression Levels and Clinical Severity of Infection in Covid-19 Patients

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dc.authoridBayyurt, Burcu/0000-0002-5618-457X
dc.authoridBakir, Mehmet/0000-0003-3702-1932
dc.contributor.authorArslan, Badel
dc.contributor.authorBaltaci, Sevgi
dc.contributor.authorBayyurt, Burcu
dc.contributor.authorSahin, Nil Ozbilum
dc.contributor.authorAkyurek, Murat Eser
dc.contributor.authorBakir, Mehmet
dc.contributor.authorArslan, Serdal
dc.date.accessioned2025-05-04T16:45:49Z
dc.date.available2025-05-04T16:45:49Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractEpigenetic modifications are known to be effective in the severity and mortality rate of SARS-CoV-2 infection. N6-methyladenosin (m6A) is a posttranscriptional modification that is carried out by m6A methyltransferases (METTL3, METTL14, and WTAP). This modification is effective in the formation of a natural immune response in the relationship between the viral genome and the host cell. In this study, the relationship between clinical severity and METTL3, METTL14, WTAP expression levels in Covid-19 patients was studied for the first time. Also, patients' D-dimer, ferritin, and C-reactive protein values were compared with these gene expression levels. Total RNA was extracted from blood samples of 100 volunteers and gene expressions were measured using a quantitative real-time polymerase chain reaction. It was determined that METTL3 (p < 0.001) and METTL14 (p = 0.005) genes were statistically significant between case and control. In addition, METTL14 (p = 0.007) and WTAP (p = 0.015) gene expressions were significantly increased in patients with severe disease. METTL14 was statistically significant between the male patients and the control (fold change = 63.87, p = 0.015). Overexpression of the METTL14 gene may have resulted in higher clinical severity in males. Our results demonstrate that host N6-methyladenosine (m6A) methyltransferases may be effective in the development of SARS-CoV-2 infection and prognosis of the disease.
dc.description.sponsorshipMersin University Scientific Research Projects; [2021-2-AP5-4537]
dc.description.sponsorshipThis study was supported by Mersin University Scientific Research Projects with the project numbered 2021-2-AP5-4537.
dc.identifier.doi10.3103/S0891416823020118
dc.identifier.endpage136
dc.identifier.issn0891-4168
dc.identifier.issn1934-841X
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85170575154
dc.identifier.scopusqualityQ4
dc.identifier.startpage129
dc.identifier.urihttps://doi.org/10.3103/S0891416823020118
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35229
dc.identifier.volume38
dc.identifier.wosWOS:001080293800010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPleiades Publishing Inc
dc.relation.ispartofMolecular Genetics Microbiology and Virology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectCOVID-19
dc.subjectN-methyladenosine
dc.subjectMethyltransferases
dc.subjectClinical severity
dc.titleRNA N6-Methyladenosine Pathway Writer Genes Expression Levels and Clinical Severity of Infection in Covid-19 Patients
dc.typeArticle

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