Experimental and theoretical observations in a mixed mode dispersive solid phase extraction of exogenous surfactants from exhaled breath condensate prior to HPLC-MS/MS analysis

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dc.authoridKatin, Konstantin/0000-0003-0225-5712
dc.contributor.authorKhoubnasabjafari, Maryam
dc.contributor.authorAltunay, Nail
dc.contributor.authorTuzen, Mustafa
dc.contributor.authorKatin, Konstantin P.
dc.contributor.authorFarajzadeh, Mir Ali
dc.contributor.authorKaya, Savas
dc.contributor.authorHosseini, Mohamadbagher
dc.date.accessioned2024-10-26T18:11:30Z
dc.date.available2024-10-26T18:11:30Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractA mixed mode dispersive solid phase extraction method was introduced for the extraction of three lung surfactants from exhaled breath condensate samples. Considering the trends to green analytical chemistry, organic polymers including polystyrene (PS), polymethylmethacrylate (PMMA-15 K), and polymethylmethacrylate (PMMA-45 K) were utilized as the sorbent for extraction of the analytes. The extraction capability for each polymer toward the studied analytes was evaluated using simplex centroid design. Based on the results, a mixture of sorbents consisting of PS, PMMA-15 K, and PMMA-45 K mixture with the mass ratio of 1:2:1: w/w/w was selected as the suitable sorbent. The effective parameters influencing the method's efficiency were investigated and optimized. Based on the figure of merit for the developed method, the calibration curves were linear in the concentration range of 0.76-10 0 0 ng mL -1 and limits of detection were from 0.09 to 0.19 ng mL -1 . The method repeatability was investigated at three concentrations as inter- and intra-day precisions and the obtained data showed that they were in the ranges of 5.2-9.1 and 4.2-8.9%, respectively. The enrichment factors were in the range of 88-100. The developed method was successfully employed in the analysis of the surfactants in the exhaled breath samples of three premature infants collected from the expiratory circuits of the mechanical ventilators. The nature of the chemical interactions with PMMA-PS complex system of the surfactants was investigated through Density Functional Theory calculations. Calculated binding energies showed that PMMA-PS complex system exhibit high performance in the extraction of lung surfactants. The most powerful interaction is between PMMA-PS complex system and 1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine.(c) 2023 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTabriz University of Medical Science [65552]
dc.description.sponsorshipThe financial support of Tabriz University of Medical Science for under grant No. of 65552 is greatly acknowledged.
dc.identifier.doi10.1016/j.molstruc.2023.135096
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopus2-s2.0-85147903165
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2023.135096
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30701
dc.identifier.volume1281
dc.identifier.wosWOS:000939669800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Molecular Structure
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHPLC-MS
dc.subjectMS
dc.subjectDispersive solid phase extraction
dc.subjectLung surfactants
dc.subjectExperimental and theoretical observations
dc.titleExperimental and theoretical observations in a mixed mode dispersive solid phase extraction of exogenous surfactants from exhaled breath condensate prior to HPLC-MS/MS analysis
dc.typeArticle

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