Acrylamide-Sepiolite Based Composite Hydrogels for Immobilization of Invertase

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dc.authoridSaraydin, Dursun -- 0000-0001-7191-510Xen_US
dc.contributor.authorOztop, H. Nursevin
dc.contributor.authorHepokur, Ceylan
dc.contributor.authorSaraydin, Dursun
dc.date.accessioned2019-07-27T12:10:23Z
dc.date.accessioned2019-07-28T10:14:06Z
dc.date.available2019-07-27T12:10:23Z
dc.date.available2019-07-28T10:14:06Z
dc.date.issued2009
dc.department[Oztop, H. Nursevin -- Hepokur, Ceylan] Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey -- [Saraydin, Dursun] Cumhuriyet Univ, Dept Chem, Hydrogel Res Lab, TR-58140 Sivas, Turkeyen_US
dc.description.abstractNovel composite hydrogels, poly(acrylamide)-sepiolite (PAS), poly(acrylamide/acrylic acid)-sepiolite (PAAS), and poly(acrylamide/itaconic acid)-sepiolite (PAIS) were prepared and used for the immobilization of invertase. The parameters of equilibrium swelling, diffusional exponent, and diffusion coefficient of these hydrogels were calculated from swelling experiments. Invertase was immobilized onto PAS, PAAS, and PAIS and immobilized invertases (PASI, PAASI, and PAISI) were prepared. Optimum pH values for free invertase, PASI, PAASI, and PAISI are found to be 5, 5.5, 4.5, and 6, respectively, and the optimum temperatures were 30, 50, 50, and 35 degrees C for free invertase PASI, PAASI, and PAISI. It was found that K-m values of free invertase, PASI, PAASI, and PAISI were 11.3, 41.0, 94.5, and 56.0 mM, respectively. V-max values were 2 mu mol/min for free invertase, 8.10 mu mol/min for PASI, 1.30 mu mol/min for PAASI, and 0.42 mu mol/min for PAISI, respectively. The invertase immobilized hydrogels showed excellent, temperature, storage, and operational stability.en_US
dc.description.sponsorshipCumhuriyet Univ. Research Funden_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial support provided by the Cumhuriyet Univ. Research Fund through Project F-203.en_US
dc.identifier.doi10.1111/j.1750-3841.2009.01302.xen_US
dc.identifier.endpageN49en_US
dc.identifier.issn0022-1147
dc.identifier.issn1750-3841
dc.identifier.issue7en_US
dc.identifier.pmid19895491en_US
dc.identifier.scopus2-s2.0-69749126253en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpageN45en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1750-3841.2009.01302.x
dc.identifier.urihttps://hdl.handle.net/20.500.12418/10069
dc.identifier.volume74en_US
dc.identifier.wosWOS:000269498300038en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofJOURNAL OF FOOD SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectacrylamideen_US
dc.subjectcompositeen_US
dc.subjectimmobilizationen_US
dc.subjectinvertaseen_US
dc.subjectsepioliteen_US
dc.titleAcrylamide-Sepiolite Based Composite Hydrogels for Immobilization of Invertaseen_US
dc.typeArticleen_US

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