Mechanism of anti-cancer effect of ?-glucan on SH-SY5Y cell line

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dc.authoridyulak, fatih/0000-0003-3708-6752
dc.authoridJoha, Ziad/0000-0001-8520-3760
dc.contributor.authorFiliz, Ahmet Kemal
dc.contributor.authorJoha, Ziad
dc.contributor.authorYulak, Fatih
dc.date.accessioned2024-10-26T18:04:18Z
dc.date.available2024-10-26T18:04:18Z
dc.date.issued2021
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAnti-cancer property of fungi derived 13-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of 13-glucan (62.5, 125, 250 and 500 pg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. 13-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that 13-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, 13-glucan treatment did not change the BCL-2 protein level. Altogether, 13-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis.
dc.identifier.doi10.3329/bjp.v16i4.54872
dc.identifier.endpage128
dc.identifier.issn1991-007X
dc.identifier.issn1991-0088
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85117473170
dc.identifier.scopusqualityQ3
dc.identifier.startpage122
dc.identifier.urihttps://doi.org/10.3329/bjp.v16i4.54872
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28862
dc.identifier.volume16
dc.identifier.wosWOS:000704886700002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherBangladesh Pharmacological Soc
dc.relation.ispartofBangladesh Journal of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleMechanism of anti-cancer effect of ?-glucan on SH-SY5Y cell line
dc.typeArticle

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