Analysis of glipizide and pravastatin sodium drug molecules at trace level by using magnetic solid phase extraction and HPLC-DAD system

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dc.contributor.authorPolat, Ummugulsum
dc.contributor.authorUlusoy, Halil brahim
dc.contributor.authorPalabiyik, Ismail Murat
dc.date.accessioned2025-05-04T16:47:10Z
dc.date.available2025-05-04T16:47:10Z
dc.date.issued2025
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAn easy applicable and selective sample preparation technique has been developed for trace and simultaneously analysis of Glipizide (GLP) and Pravastatin (PST) molecules in biological matrices based on magnetic solid phase extraction (MSPE) and high-performance liquid chromatography (HPLC). A new magnetic adsorbent including Fe3O4@TEOS-Melamine has been synthetized and characterized for extraction studies. Experimental variables of MSPE were examined and optimized step by step such as pH, adsorption and desorption conditions, time effect, etc. Simultaneous HPLC analysis of GLP and PST molecules was performed by isocratic elution of a mixture of acetonitrile: phosphate buffer (pH:3.0, 0.02 M) (35:65) with flow rate 1.0 mL min- 1. Analytical signals obtained from DAD detector were recorder in 238 and 226 nm for PST and GLP, respectively. The limit of detections (LOD) for proposed method were 3.17 ng mL- 1 for PST and 3.03 ng mL- 1 for GLP molecules. Consequently, accuracy and precision of developed method were tested by means of recovery tests in synthetic urine and saliva samples. RSD % values were lower than 6.1 % and recovery values were in the range of 98.5-103.3 % for both molecules.
dc.description.sponsorshipSivas Cumhuriyet University Scientific Research Projects Commission [ECZ-2021-082, ECZ-2023-093]
dc.description.sponsorshipThis study was created by using experimental data obtained from Ummugulsum Polat's PhD thesis and partly supported by Sivas Cumhuriyet University Scientific Research Projects Commission by means of research projects (ECZ-2021-082 and ECZ-2023-093). The authors thank Gokhan Sarp for his help in characterization analysis of magnetic particles. A part of experimental data in this study was submitted in 33rd International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2023) as an oral presentation.
dc.identifier.doi10.1016/j.jchromb.2025.124477
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid39869996
dc.identifier.scopus2-s2.0-85215957372
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2025.124477
dc.identifier.urihttps://hdl.handle.net/20.500.12418/35516
dc.identifier.volume1253
dc.identifier.wosWOS:001412799500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250504
dc.subjectPravastatin sodium
dc.subjectGlipizide
dc.subjectMagnetic solid phase extraction
dc.subjectUrine
dc.subjectSaliva
dc.subjectHPLC
dc.titleAnalysis of glipizide and pravastatin sodium drug molecules at trace level by using magnetic solid phase extraction and HPLC-DAD system
dc.typeArticle

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