Anticancer activity, hTERT expression and telomere length analysis in SH-SY5Y neuroblastoma cell lines applied to docetaxel

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dc.authorid0000-0002-0562-7457tr
dc.contributor.authorInandiklioglu, Nihal
dc.contributor.authorTas, Ayca
dc.contributor.authorAgbektas, Tugba
dc.contributor.authorTuncbilek, Zuhal
dc.contributor.authorRaheem, Kayode Yomi
dc.contributor.authorCinar, Gulcihan
dc.contributor.authorSilig, Yavuz
dc.date.accessioned2024-03-07T05:55:27Z
dc.date.available2024-03-07T05:55:27Z
dc.date.issued05.02.2023tr
dc.departmentTıp Fakültesitr
dc.description.abstractNeuroblastoma is the most common extracranial solid tumor of infancy in a broad range of clinical courses, ranging from spontaneous regression to fatal progression. Telomere maintenance plays an impor- tant role in genome stability and cell proliferation. Telomerase reverse transcriptase (hTERT in humans) is a catalytic subunit of the enzyme telomerase. In this study, it was aimed to determine the anticancer ac- tivity of the docetaxel chemotherapeutic agent in neuroblastoma cell line (SH-SY5Y) and to investigate its effect on hTERT gene expression level and telomere length. Molecular docking studies were performed on docetaxel with the crystal structure of telomerase. The electronic properties of docetaxel were calculated using the density functional theory (DFT) method. SH-SY5Y cells were treated for 24, 48 and 72 h with specific concentrations of docetaxel drug ranging from 1 to 100 μg/ml. IC50 doses of docetaxel were de- termined and administered to SH-SY5Y cells, followed by RNA isolation. hTERT and MYC gene expression levels and telomere length were measured in the docetaxel-treated sample using the RT-PCR method. In addition, theoretical analyzes were made. The IC50 values of docetaxel after 24, 48 and 72 h were 8.32 ±1.45 μg/ml, 7.67 ±2.56 μg/ml and 5.51 ±1.24 μg/ml, respectively. According to the results obtained, docetaxel was found to have the highest activity in 72 h of incubation. It was determined that the do- cetaxel drug decreased the expression level of the hTERT gene in SH-SY5Y cells. Telomere lengths were significantly reduced in the docetaxel treated SH-SY5Y cell line compared to the control group ( p < 0.05). Molecular docking analysis results were in agreement with the experiments. Analysis results indicated a good interaction between docetaxel and the active site of telomerase. The results of this study, reinforced by molecular docking analyzes, might be proved valuable for the development of potent telomerase in- hibitors.tr
dc.identifier.doi10.1016/j.molstruc.2022.134346en_US
dc.identifier.issn0022-2860
dc.identifier.scopus2-s2.0-85140095305en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage134346tr
dc.identifier.urihttps://hdl.handle.net/20.500.12418/14828
dc.identifier.volume1273tr
dc.identifier.wosWOS:000904602100012en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElseviertr
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/closedAccesstr
dc.subjectDocetaxeltr
dc.subjectMolecular dockingtr
dc.subjectNeuroblastomatr
dc.subjectTelomerasetr
dc.subjectTelomere lengthtr
dc.titleAnticancer activity, hTERT expression and telomere length analysis in SH-SY5Y neuroblastoma cell lines applied to docetaxelen_US
dc.typeArticleen_US

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