Bentonite-acrylamide-histidine-Cu(II) Microcomposite for Cytochrome c Adsorption

Bentonite-acrylamide-histidine with an average particle diameter of 200-1000 mu m was produced by bulk polymerization of bentonite, acrylamide, bisacrylamide, AIBN and histidine. Bentonite-acrylamide-histidine-Cu(II) microcomposite was prepared by loading copper ions. The microcomposite was characterized by scanning electron microscopy (SEM), specific surface area and elemental analysis. Cytochrome c adsorption studies were performed, for the first time, by using the new metal chelated affinity support (bentonite-acrylamide-histidine-Cu(II)) microcomposite in batchwise experiments.