Molecular diversity of Klebsiella pneumoniae clinical isolates: antimicrobial resistance, virulence, and biofilm formation

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dc.authoridhasbek, mursit/0000-0002-5217-8607
dc.authoridDurna Dastan, Sevgi/0000-0003-4946-5602
dc.authoridCELIK, CEM/0000-0002-7141-5874
dc.authoridASLAN, RUKIYE/0000-0001-5843-626X
dc.contributor.authorKafa, Ayse Humeyra Taskin
dc.contributor.authorAslan, Rukiye
dc.contributor.authorDastan, Sevgi Durna
dc.contributor.authorCelik, Cem
dc.contributor.authorHasbek, Mursit
dc.contributor.authorEminoglu, Aysenur
dc.date.accessioned2024-10-26T18:07:44Z
dc.date.available2024-10-26T18:07:44Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractOne of the mechanisms responsible for antibiotic resistance in Klebsiella pneumoniae is the enzymes produced by the bacteria; another important mechanism is the ability to form biofilm. In this study, antibiotic resistance, genes associated with virulence, and biofilm-forming properties of K. pneumoniae strains were investigated. A total of 100 K. pneumoniae isolates were obtained from different clinical samples identified by Matrix-Assisted Laser Desorption/Ionization time-of-flight Mass Spectrometry. Antimicrobial susceptibility testing was performed with the Phoenix 100 apparatus. The biofilm forming properties of strains were determined by the microtiter plate method. For molecular analysis, genes encoding the carbapenemase enzyme (bla(OXA-48), bla(NDM-1), bla(IMP), and bla(VIM)) and biofilm-related genes (treC, luxS, mrkA, and wza) were investigated by polymerase chain reaction (PCR). While 76% of clinical isolates were resistant to three or more antimicrobials, 24% were classified as non-multidrug resistant (non-MDR). When biofilm-forming capacities of clinical isolates were tested, it was determined that the resistant-isolates produced 59.2% strong biofilm, and susceptible-isolates produced 12.5% strong biofilm. According to PCR results, carbapenemase genes were determined as follows: bla(OXA-48)-70%, bla(NDM)-49%, and bla(KPC)-19%, bla(OXA-48)/bla(NDM)/bla(KPC)-12%, bla(OXA-48)/bla(NDM)-26%, and bla(OXA-48)/bla(KPC)-4%. The biofilm-associated genes in bacterial isolates were determined as follows: luxS-98%, treC-94%, mrkA-88%, and wza-15%. In addition, Hierarchical Clustering Tree and Heatmap analysis revealed an association between isolates that lacks resistance genes and isolates lacks biofilm-formation related genes that were included in MDR or non-MDR classes. As a result, biofilm should be considered in the treatment of MDR infections, and therapy should be planned accordingly. In addition, pursuing the data and genes of antibiotic resistance is significant for combating resistance.
dc.identifier.doi10.1080/15257770.2024.2344741
dc.identifier.issn1525-7770
dc.identifier.issn1532-2335
dc.identifier.pmid38718417
dc.identifier.scopus2-s2.0-85192566898
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1080/15257770.2024.2344741
dc.identifier.urihttps://hdl.handle.net/20.500.12418/29661
dc.identifier.wosWOS:001219623100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofNucleosides Nucleotides & Nucleic Acids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectKlebsiella pneumoniae
dc.subjectantibiotic resistance
dc.subjectcarbapenemase
dc.subjectbiofilm
dc.titleMolecular diversity of Klebsiella pneumoniae clinical isolates: antimicrobial resistance, virulence, and biofilm formation
dc.typeArticle

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