Experimental and Theoretical Evidence on the Amoebicidal Activity of Synthesized tRNA-Palmitic Acid Esters

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dc.contributor.authorAksu, Aysun
dc.contributor.authorCelik, Muhammed Safa
dc.contributor.authorPolat, Zubeyde Akin
dc.contributor.authorYenidunya, Ali Fazil
dc.contributor.authorCetinkaya, Serap
dc.contributor.authorTuzun, Burak
dc.date.accessioned2024-10-26T18:03:30Z
dc.date.available2024-10-26T18:03:30Z
dc.date.issued2024
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractSynthesis of tRNA-palmitic acid esters was carried out in dimethyl sulfoxide (DMSO) and tert-butanol, %20 and %80 (v/v), respectively, for 24h at 55oC. These conditions have been to be optimal in the synthesis of monosaccharide-fatty acid esters in previous studies. The majority of the ester product (89.19%) was phthalic acid, butyl undecyl ester, as identified by GC-MS. This finding hinted that the ester bond took place between tRNA ribose unit(s) and carboxyl group of the palmitic acid. NMR (Nuclear Magnetic Resonance) was also used for the characterization of the ester product (data not shown). The ester product (O1) exerted remarkable amoebicidal activity, at 4mg/ml, on the trophozoites and cysts of Acanthamoeba castellanii. No live trophozoites or cysts could be detected after the 24th and 72th of incubation, respectively. The study included all the necessary control experiments. Interactions between the substrate molecules were scrutinized by molecular docking calculations. Activities of the studied molecules against various Acanthamoeba castellanii proteins (PDB ID: 1PRQ and 6UX0), in crystal form, were compared, and finally, ADME/T analyses were carried out.
dc.description.sponsorshipScientific Research Project Fund of Sivas Cumhuriyet University (CUBAP) [RGD-020]; Sivas Cumhuriyet University Scientific Research Projects, Turkey [F-617]
dc.description.sponsorshipThe numerical calculations reported in this paper were fully/partially performed at TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources) . This work was supported by the Scientific Research Project Fund of Sivas Cumhuriyet University (CUBAP) under the project number RGD-020. The study was funded by the Sivas Cumhuriyet University Scientific Research Projects, Turkey (No. F-617) .
dc.identifier.endpage706
dc.identifier.issn1021-9986
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85201633362
dc.identifier.scopusqualityQ3
dc.identifier.startpage695
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28377
dc.identifier.volume43
dc.identifier.wosWOS:001314983700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJihad Daneshgahi
dc.relation.ispartofIranian Journal of Chemistry & Chemical Engineering-International English Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcanthamoeba castellanii
dc.subjectAmoebicidal activity
dc.subjectSynthesis
dc.subjecttRNA-fatty acid ester
dc.subjectMolecular docking
dc.titleExperimental and Theoretical Evidence on the Amoebicidal Activity of Synthesized tRNA-Palmitic Acid Esters
dc.typeArticle

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