The Anticonvulsant Effects of Alpha-2 Adrenoceptor Agonist Dexmedetomidine on Pentylenetetrazole-Induced Seizures in Rats

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dc.authoridOzdemir, Ercan/0000-0001-8231-1053
dc.authoridTaskiran, Ahmet Sevki/0000-0002-5810-8415
dc.contributor.authorCetindag Ciltas, Arzuhan
dc.contributor.authorOzdemir, Ercan
dc.contributor.authorGumus, Erkan
dc.contributor.authorTaskiran, Ahmet Sevki
dc.contributor.authorGunes, Handan
dc.contributor.authorArslan, Gokhan
dc.date.accessioned2024-10-26T18:09:43Z
dc.date.available2024-10-26T18:09:43Z
dc.date.issued2022
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractAlpha2-adrenoreceptor (alpha(2)-AR) is a noradrenergic receptor that is frequently studied for modulation of seizure activity. However, the precise role of this receptor agonists in regulating seizure activity is still unclear. Our aim in this study was to investigate the effects of alpha(2)-AR agonist dexmedetomidine (DEX) and atipamezole (alpha(2)-AR antagonist, ATI) on seziures in rats. In the study, 32 adult male Wistar Albino rats (weighing 220-260 g) were used. To induce seizures in rats, pentylenetetrazole (PTZ, 35 mg/kg) was injected intraperitoneally (i.p.) and seizure stages were determined according to the Racine scale. After induction of seizures, DEX (0.1 mg/kg, i.p.) and ATI (1 mg/kg, i.p.) were administered to rats and their effects determined on seizures. GABA levels of the brain hippocampal tissue sample were measured using an ELISA kit and c-Fos positive cells of the dentate gyrus and hippocampal regions were quantitatively analyzed with Image J software. The results showed that DEX decreased the seizure stages according to the Racine scale, significantly prolonged the onset time of first myoclonic jerk (FMJ) and reduced the number of spikes and percentage seizure duration (p < 0.05). In contrast, ATI increased the seizure stage, the number of spikes and percentage seizure duration. The hippocampal GABA level was significantly decreased in rats with only PTZ injection (p < 0.05). In addition, DEX reduced the number of c-Fos positive cells in dentate gyrus and the hippocampal CA1 and CA3 regions. In conclusion, our findings showed that alpha 2-AR agonist DEX had a reducing activity on PTZ-induced seizure, while alpha 2-AR antagonist ATI facilitated seizure formation.
dc.description.sponsorshipCumhuriyet University Scientific Research Project, Sivas, Turkey (CUBAP) [T-718]
dc.description.sponsorshipThis study was supported by Cumhuriyet University Scientific Research Project, Sivas, Turkey (CUBAP, Grant Number: T-718).
dc.identifier.doi10.1007/s11064-021-03445-4
dc.identifier.endpage314
dc.identifier.issn0364-3190
dc.identifier.issn1573-6903
dc.identifier.issue2
dc.identifier.pmid34491515
dc.identifier.scopus2-s2.0-85114379664
dc.identifier.scopusqualityQ1
dc.identifier.startpage305
dc.identifier.urihttps://doi.org/10.1007/s11064-021-03445-4
dc.identifier.urihttps://hdl.handle.net/20.500.12418/30246
dc.identifier.volume47
dc.identifier.wosWOS:000695347800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer/Plenum Publishers
dc.relation.ispartofNeurochemical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpilepsy
dc.subjectDexmedetomidine
dc.subjectAtipamezole
dc.subjectc-Fos
dc.subjectGABA
dc.titleThe Anticonvulsant Effects of Alpha-2 Adrenoceptor Agonist Dexmedetomidine on Pentylenetetrazole-Induced Seizures in Rats
dc.typeArticle

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